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时间:2020-05-03
《反义RNA寡核苷酸及其与脂质体的复合物对于乙型肝炎病毒的抑制-论文.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、II3I3⋯cs、)l,、)nv.pibb.ac.cnInhibitionofHepatitisBVirusinMicebyCationicLiposome/RNAOlig0nucle0tideComplex术ZANYan-Lu一.TIANPo)”(CenterforMolecularVirology,CASKeyLaboratoryofPathogenicMicrobiologyandImmunologyInstituteofMicrobiology,ChineseAcademyofSciences,Beijing100101,Ch
2、ina;UniversityofChineseAcademyofSciences,Beijing100101,China)AbstractWeinvestigatedtheinhibitoryeffectofmodifiedantisenseRNAoligonucleotidesandcationicliposome.RNAcomplexesontherepressionofhepatitisBvirus(耶V)replicationandexpression.ELISAandquantitativeReal—timePCRanaly
3、sisshowedthatHBVreplicationandantigensexpressionbothinpHBV1.3transducedHepG2andHe0G2.2.15cellswerereducedaftertreatmentwithantisenseRNAoligonucleotidesP-2987,X一60andX.519.Subsequently.theantisenseRNAoligonucleotidesandcontrolRNAoligonucleotideswereinjectedviathetailvein
4、intoHBVtransgenicmiceorhydrodynamicallyinjectedmice.IntheHBVtransgenicmice,withthetreatmentofX·519,HBVpregenomicRNAintheliverdecreasedby81%.Cationic1iposomefi,Lrtherincreasedtheinhibitionefectoryto91%.ButnosignificantdiferenceswereobservedforHBVantigensandHBVDNAcopynumb
5、erinthesera.InacuteHBViIlfectionmousemodelbyhydrodynamicinjection.ELISAandquantitativerea1.timePCRanalysisshowedthatX.519significantlyrepressedHBVreplication,asmeasuredbyHBVpregenomicRNA,antigensexpression,andpresenceofHBVDNAinthesera.Takentogether,thesynthesizedantisen
6、seRNAoligonucleotideX一519repressed皿Vreplicationandantigensexpressioninvitroandiniv0.KeywordshepatitisBvires,antisenseRNAoligonucleotides,HBVtransgenicmice,hydrodynamicinjection,cationicliposome—RNAcomplexDoI:10.3724/SP.J.1206.2013.00184HepatitisBvirus(HBV)istheprototypi
7、ca1RNAservesasanmRNAbothfortheviralcorememberoftheHepadnaviridae(hepatotropicDNAproteinandfortheviralpolymerase.virus)family.HBVvirionsaredouble—shelledHBVinfection.whichcancauseacuteorchronicparticles,withanouterlipoproteinenvelopethathepatitis,cirrhosis,andhepatocellu
8、larcarcinoma.isacontainsthreerelatedenvelopeglycoproteins.Thecommonhealthburdenaroundtheworld,especiallyinHBVg
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