实验室常用的技术参考资料(建议打印出来).doc

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1、核酸分子量换算1basent(dNMP)=325Da1basepairnt(dsDNA)=650Da1kbssDNA二330kDa1kbdsDNA=660kDa1kbssRXA=340kDa1mgof1kbDNA=1.52pmolpmolof1kbDNA0.66mg核酸（UV吸光值）dsDNA:A260=50mg/mlssDNA:A260=33mg/mlssRXA:A260=40mg/mlp.s.這些值是在微鹼性的環境下通過1cm光徑所估計的抽取出的純DNAA260/A280應該接近1.8,<1.7表示有些微蛋白質污染；純R

2、XA則應該接近2.0。常用同位素的性质NuclideHatf-lifetiEmissioEnergy,mRangeofemisJF■■■■■•Shield(halfvaluelayer)menax.(Mevjsion,max・H312.43ryeap0.01860.42cm(air)-C145370yearp0.15621.8cm(air)-p3214.3dayp1.71610cm(air)Acrylicglass(1cm)0.8cm(water)0.76cm(acrylicglas)p3325.4dayp0.24949cm

3、(air)Acrylicglass(1cm)S3587.4dayp0.16724.4cm(air)-

4、12560day•4A0.27~0.0350.2mm(lead)Lead(0.02mm)常见的酸和碱ChinesenameMolecularFormulaMolecularweight%byweightMolarity(M)1Msolution(ml/L)specificgravityAcidsAceticacid冰醋酸CH3COOH6005996174575105Formicacid甲酸、蟻酸HCOOH4603909823

5、625.942.438.51205122Hydrochloricacid鹽酸HC136463611.6859118Nitricacid硝酸HNO363017015.763.71.42Perchloricacid過氯酸HC10-100.4660729.2122108.88211.541.70Phosphoricacid磷酸H3PO498008514.7678170Sulfuricacid硫酸H:SO-98.079818.354.51.835BasesAmmoniumhydroxide氫氧化錶、氨水NH4OH35.02814.8

6、67609Potassiumhydroxide氫氧化鉀KOH56114511.68221447Sodiumhydroxide氫氧化銷NaOH40.050191524153ATP等常见物质的分子量CompoundMolecularweight(Da)ATP507.2CTP483.2GTP523.2UTP484.2dATP491.2dCTP467.2dGTP507.2dTTP482.2AMP347.2CMP323.2GMP363.2UMP324.2dAMP312.2dCMP288.2dGMP328.2dTMP303.2琼脂糖凝胶

7、DNA分辨率Gel%DNAsizerange0.5%0.7%1.0%1.2%1.5%3-4%sievedagarose1-30kb0.8〜12kb0.5-10kb0.4-7kb0.2~3kb0.01~1kb聚丙烯酰胺凝胶DNA分辨率Gel%DNAsizerange3.5%5.0%8.0%12.0%15.0%20.0%1000-2000bp75-500bp50~400bp35-250bp20-150bp5"00bp聚丙烯酰胺凝胶蛋白质分辨率Gel%Proteinsizerange5.0%57-212kDa7.5%36〜94kD

8、a10.0%16〜68kDa15.0%12~43kDa常用蛋白酶抑制剂的使用浓度InhibitorSpecificityofinhibitionSolubleSuggeste(!进伽gconceAEBSF(ArninoethylSerineproteases:trypsin,H2Obenzylsulfonylfluorichymotrypsin,plasmin,plde)asmakallikrein,thrombin.EtOHItisawater-solublesubstituteforPMSFwithamuchlonger

9、half-lifeinaqueoussolutionAntipain-dihydrochpapain.,trysin,cathepsinH2OlorideAandBO.l^l.Omg/ml(0.4~4mM)AprotininSerineproteases:trypsin,chymotryp