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1、《EnglishforBiologyStudents》、atfirstglance,It'saprettydauntingsubject.However,undertheleadershipoftheteacherZhang,weenjoyedit!Now,gobackandhavealookatthewaywehavegothrought,butfindwithlove.TobeginningwiththenfearnandthennrelievedH,butendwithHloveH.《EnglishforBiologyStudents》broughtmealotoffii
2、n,sentimentandhelp・TeacherZhangnotonlygiveustheprofessionalEnglishknowledge,whafsmore,healsogaveusalotofknowledgethoseoutsideoftextbooks・Heisanexcellentprofessionalteacher,Hemademeunderstandthoughexcellentprofessionallevelisimportant,comprehensiveabilityandbroadenourknowledgeisindispcnsablc.
3、Hismanyrichinphilosophy,stillsovividinmymind!"Summaryortheabbreviation0chooseonefromthetwotodoaslastjobabouttheBiologicalprofessionalEnglish.OriginallyIthought:abbreviation.ReadanEnglishliterature,andthenalittleunderstanding,abbreviationswouldnotbeaproblem・However,Istillwanttochallengemyself
4、.Notseekingcomparedwithothers,buttosurpassmyself!SodecisivelyIchoosealargerreviewoftheHriskn—summary.Fortunately,sophomoreyear,IservedastheMinisterofScienceandTechnologyofLifeScience,Ihavearchivedpapersofallresearchprojects・Ofcourse,thesepapersofaresearchprojectsIwouldn'trumor.Ionlyreferenee
5、themtopracticemyEnglishskills.ThesiteandthemechanismofoxidationofArgininekinaseAbstractArgininekinase(argininekinase,AK)(EC2.7.3.3)isaphosphodiesteraseguanidineskinase,composedof403aminoacids,whichcontainsfivecysteines(Cys23,Cysl27,Cysl39,Cys201,Cys271),theexperimentwcfoundthattheoxidationst
6、atebandandrestorethestatewiththephenomenonofelectrophoresisAK,andthroughaseriesofbiotechnologymeans,respectively,ofthesefivesitesCysmutatedtoserine(Ser),toidentifyoxidationsitesandtostudytheoxidationmechanism,andbyUVfluorescence,respectively,toexplorethestructureafterthemutationofthetheAKvar
7、ioussitesrelatedtoactivity.Keywords:Argininekinase,rite-directedmutation,oxidationsites,SDS-PAGEIntroductionl.Argininekinase[l](ArgininekinaseAK)(EC2.7.3.3)isakinaseofaphosphorylatedoriginalguanidinocompoundexistswidelyininvertebrates,andisanimport