资源描述:
《Molecular and Cellular Approaches for Diversifying and Extending Optogenetics 》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、ResourceMolecularandCellularApproachesforDiversifyingandExtendingOptogeneticsVivianaGradinaru,1,2,6FengZhang,1,5,6CharuRamakrishnan,1JoannaMattis,1,2RohitPrakash,1,2IlkaDiester,1InbalGoshen,1KimberlyR.Thompson,1andKarlDeisseroth1,3,4,*1DepartmentofBioengineering2PrograminNeuroscience3Departmentof
2、PsychiatryandBehavioralSciences4HowardHughesMedicalInstituteStanfordUniversity,Stanford,CA94305,USA5Presentaddress:SocietyofFellows,HarvardUniversity,Cambridge,MA02138,USA6Theseauthorscontributedequallytothiswork*Correspondence:deissero@stanford.eduDOI10.1016/j.cell.2010.02.037SUMMARYPoo,1998;Sil
3、berbergetal.,2004)canchangethemagnitudeorflipthesignofneuronalactiononthecircuit.Similarly,pancre-Optogenetictechnologiesemploylighttocontrolaticbcellsexecutesynchronizedoscillationsinmembranebiologicalprocesseswithintargetedcellsinvivopotentialresultinginpulsatileinsulinsecretionwithprecisewithhi
4、ghtemporalprecision.Here,weshowthattimingacrossthepopulationofbcellsthatmaybeimportantapplicationofmoleculartraffickingprinciplescanfortheactionofinsulinontargettissues(TengholmandGylfe,expandtheoptogeneticrepertoirealongseveral2009).Traditionalgeneticsdeliverscelltype-specificcontrolifadequatetarg
5、etingstrategiesexist,butgeneticapproacheslong-soughtdimensions.Subcellularandtranscellu-lackthetemporalprecisionneededtocontroleventswithlartraffickingstrategiesnowpermit(1)opticalregu-behaviorallyorenvironmentallyrelevanttriggeringandtiming;lationatthefar-red/infraredborderandextensionmoreover,ph
6、armacologicalcontrolstrategieslackcelltype-ofoptogeneticcontrolacrosstheentirevisiblespec-specificity,temporalprecision,orboth.Thegeneralneedtotrum,(2)increasedpotencyofopticalinhibitionstudymolecularandcellulareventsnotonlyinreducedsystems,withoutincreasedlightpowerrequirement(nanoam-butalsowithi
7、nintactbiologicalsystems,hasdrivenrecentpere-scalechloride-mediatedphotocurrentsthatawarenessoftheopportunitiesoffastcontrol.maintainthelightsensitivityandreversible,step-Toenabletemporallyprecisecontrolofspecificcellty