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1、PLVX-Puro载体信息如下：pLVXpuro载体基本信息出品公司:Clontech载体名称:pLVX-Puro,pLVXpuro质粒类型:哺乳动物细胞慢病毒表达载体高拷贝/低拷贝:高拷贝启动子:CMV克隆方法:多克隆位点，限制性内切酶载体大小:8102bp5'测序引物及序列:CMV-F：CGCAAATGGGCGGTAGGCGTG3'测序引物及序列:--载体标签:--载体抗性:氨苄筛选标记:嘌呤霉素备注:含有组成型CMV启动子的慢病毒载体产品目录号:632164稳定性:/组成型:--病毒/非病毒:慢病毒pLVXpuro
2、载体质粒图谱和多克隆位点信息pLVXpuro载体简介DescriptionpLVX-PuroisanHIV-1-based,lentiviralexpressionvector.Lentiviralparticlesderivedfromthevectorallowyoutoexpressyourgeneofinterestinvirtuallyanycelltype,evenprimarycells.Expressionofyourgeneisdrivenbytheconstitutivelyactivehumancy
3、tomegalovirusimmediateearlypromoter(PCMVIE),locatedjustupstreamofthemultiplecloningsite(MCS),allowingconstitutive,highlevelexpressionofyourproteinofinterest.pLVX-Purocontainsalloftheviralprocessingelementsnecessaryfortheproductionofreplication-incompetentlentivir
4、us,aswellaselementstoimproveviraltiter,transgeneexpression,andoverallvectorfunction.Thewoodchuckhepatitisvirusposttranscriptionalregulatoryelement(WPRE)promotesRNAprocessingeventsandenhancesnuclearexportofviralandtransgeneRNA(1),leadingtoincreasedviraltitersfromp
5、ackagingcells,andenhancedexpressionofyourgeneofinterestintargetcells.Inaddition,thevectorincludesaRev-responseelement(RRE),whichfurtherincreasesviraltitersbyenhancingthetransportofunsplicedviralRNAoutofthenucleus(2).Finally,pLVX-Puroalsocontainsacentralpolypurine
6、tract(cPPT)elementthatincreasesnuclearimportationoftheviralgenomeduringtargetcellinfection,resultinginimprovedvectorintegrationandmoreefficienttransduction(3).Inadditiontolentiviralelements,pLVX-Purocontainsapuromycinresistancegene(Puror)underthecontrolofthemurin
7、ephosphoglyceratekinase(PGK)promoter(PPGK)fortheselectionofstabletransductants.ThevectoralsocontainsapUCoriginofreplicationandanE.coliampicillinresistancegene(Ampr)forpropagationandselectioninbacteria.UsepLVX-PuroconstitutivelyexpressesyourgeneofinterestfromPCMVI
8、Ewhentransducedintotargetcells.Beforethevectorcanbetransducedintocells,however,itmustbetransfectedinto293TpackagingcellswithourLenti-X™HTPackagingSystem(Cat.No
