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ID:37124136
大小:1.38 MB
页数:37页
时间:2019-05-18
《难治性癫痫患者颞叶皮质中NCAM140kDa的表达》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、重庆医科大学硕士研究生学位论文难治性癫痫患者颞叶皮质中NCAM.140kDa的表达摘要目的:研究难治性颞叶癫痫患者颞叶皮质中NCAM.140kDa(神经细胞粘附分子140kDa)的表达,探讨其在难治性癫痫形成中的意义,并初步探讨颞叶皮质的可塑性与难治性癫痫的关系。方法:按随机化原则,在我们建立的难治性癫痫患者术后脑组织库中抽取20例患者的颞叶皮质,用免疫组化、免疫荧光法分别检测其NCAM一140kDa的表达,并与对照组进行比较。结果:免疫组化检测到的实验组和对照组的NCAM一140k_Da平均光密度值分别
2、为:0.2290-a:0.0427、O.1789+0.0258,两组比较差异有显著性(P3、SSl0N0FNCAM.140kDaINTEMPORALLOBECORTEXOFPATIENTSWITHINTRACTABLEEPILEPSYABSTRACTObjeetive:ToinvestigatetheexpressionofNCAM-140kDainthetemporallobeneocortexofpatientswithintractabletemporallobeepilepsyanddiscussitsrolesintheformationofintractableepilepsy.Me4、thod:20casesofbraintissuesofintractableepilepsyand14casesofnonepilepticbraintissueswerecollected.TheexpressionofNCAM一140kDainthespecimenswereexaminedthroughimmunohistochemistryandindirectimmunofluorescence.Results:theNCAM-140kDastainingintensityofimmunohi5、stochemistryintheintractableepileptictemporallobetissueandthecontrolwere0.2290-a:0.0427、O.1789+0.0258respectively(P<0.05);andtheintensityofimmunofluorescencewere28.8345士3.2635、20.45l3士2.2834respectively(P<0.05).Conelusion:TheexpressionofNCAM一140kDahasasig6、nificantincreaseintheintractableepileptictemporallobecortex,suggestitmightplayaroleintheformationofintractableepilepsyo●一Keywords:intractableepilepsy,NCAM-140kDa,immunohistochemistry,immunofluorescence4重庆医科大学硕士研究生学位论文英汉缩略语名词对照英文缩写英文全称NCAMNeuralcelladhesio7、nmoleculeODopticaldensitySABCDABFITCPBShSGSCPSGTCSSECBZVPATPMLTGPBPHTOXCCLOGBPPSACA3IgC3中文全称神经细胞粘附分子光密度值链霉亲和素.生物素.过氧化Streptavidin.biotinperoxidasecomplex物酶复合物Diaminobenzidine二氨基联苯胺Fluoresceinisothiocyanate异硫氰酸荧光素phosphatebufferedsolution磷酸盐缓冲溶液hour小时Secon8、darilygeneralizedseizure继发全身性发作Complexpartialseizure单纯部分性发作Generalizedtonic—clonicseizure全身强直一阵挛性发作Statusepilepsy癫痫持续状态carbamazepine卡马西平Valproate丙戊酸Topiramate托吡酯Lamotrigine拉莫三嗪Phenobarbital苯巴比妥Phenytoin苯妥英钠Oxcarbaze
3、SSl0N0FNCAM.140kDaINTEMPORALLOBECORTEXOFPATIENTSWITHINTRACTABLEEPILEPSYABSTRACTObjeetive:ToinvestigatetheexpressionofNCAM-140kDainthetemporallobeneocortexofpatientswithintractabletemporallobeepilepsyanddiscussitsrolesintheformationofintractableepilepsy.Me
4、thod:20casesofbraintissuesofintractableepilepsyand14casesofnonepilepticbraintissueswerecollected.TheexpressionofNCAM一140kDainthespecimenswereexaminedthroughimmunohistochemistryandindirectimmunofluorescence.Results:theNCAM-140kDastainingintensityofimmunohi
5、stochemistryintheintractableepileptictemporallobetissueandthecontrolwere0.2290-a:0.0427、O.1789+0.0258respectively(P<0.05);andtheintensityofimmunofluorescencewere28.8345士3.2635、20.45l3士2.2834respectively(P<0.05).Conelusion:TheexpressionofNCAM一140kDahasasig
6、nificantincreaseintheintractableepileptictemporallobecortex,suggestitmightplayaroleintheformationofintractableepilepsyo●一Keywords:intractableepilepsy,NCAM-140kDa,immunohistochemistry,immunofluorescence4重庆医科大学硕士研究生学位论文英汉缩略语名词对照英文缩写英文全称NCAMNeuralcelladhesio
7、nmoleculeODopticaldensitySABCDABFITCPBShSGSCPSGTCSSECBZVPATPMLTGPBPHTOXCCLOGBPPSACA3IgC3中文全称神经细胞粘附分子光密度值链霉亲和素.生物素.过氧化Streptavidin.biotinperoxidasecomplex物酶复合物Diaminobenzidine二氨基联苯胺Fluoresceinisothiocyanate异硫氰酸荧光素phosphatebufferedsolution磷酸盐缓冲溶液hour小时Secon
8、darilygeneralizedseizure继发全身性发作Complexpartialseizure单纯部分性发作Generalizedtonic—clonicseizure全身强直一阵挛性发作Statusepilepsy癫痫持续状态carbamazepine卡马西平Valproate丙戊酸Topiramate托吡酯Lamotrigine拉莫三嗪Phenobarbital苯巴比妥Phenytoin苯妥英钠Oxcarbaze
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