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1、2001;21(10):721-723第一军医大学学报(JFirstMilMedUniv)721人发角蛋白人工健体内降解的形态学及泛肤、溶酶体酶的活性变化朴英杰’,童为人2,胡庆柳’,蒋东萍2傅文玉’,妻丽2,乔东访’(’第一军医大学解剖教研室,“组织学与胚胎学教研室,广东广州510515)摘要目的阐明人发角蛋白(HHK)人工键植人体内后的降解过程‘方法选取30只新西兰大自兔随机分为术后第t,3,6,9,12周实验组和正常对照组。实验组行跟胜切除后植人HHK人工脸,按期进行常规形态学观察和泛肚组化及酸性磷酸酶(AcP)酶细胞化
2、学观察。结果光镜形态学观察显示,人工皮植人后第1周出现人发毛小皮脱落、消失.人发早均质状表面附着巨噬细胞和多核巨细胞.到第3-6周可见降解成颗粒的人工服被巨噬细胞和多核巨细胞吞噬泛肚酶组化显示第卜3周人发周围的L噬细胞、多核巨细胞和成腆细胞内反应呈强阳性,周围组织呈中等阳性到第9周大部分人发被降解,泛肤酶反应在基质呈弱阳性,在眺细胞中呈中等阳性。电镜形态学观察显示毛发之间出现成破细胞,并开始分泌蛋白多糖和前胶原蛋白,第9-12周,人工胜基本被降解,同时完成了新生自体脱的形成。酶细胞化学观察显示被吞噬的颗粒呈AcP酶反应阳性。结
3、论在HHK人工胜的降解过程中泛肤系统首先在细胞外将大体积的人发降解.降解后期细胞内泛肤系统通路和溶酶体通路分别对吞入的人工胜颗粒进行降解,且具有协同作用关健词:人工器官;键角蛋白;肤类;溶酶体;酸性磷酸酶中1#1Rttt号:8318.1:8322.74文献标识码:A文童编号:1000-2588(2001)10-0721-03Morphologyofthedegradationofhumanhairkeratinartificialtendoninvivoandactivitiesofubiquitinandlysosomale
4、nzymeinthisprocessPIAOYing-jie',DONGWei-ren',HUQing-liu',jIANGDong-pine,FUWen-yu',LOULi=,QIAODong-fang'('DepartmentofAnatomy,'DepartmentofHistologyandEmbryology,FirstMilitaryMedicalUniversity,Guangzhou510515,China)Abstract:ObjectiveTounderstandtheinvivadegradationpr
5、ocessofhumanhairkeratin(HHK)artificialtendon.MethodAtotalof30NewZealandWhiteRabbitswererandomlyassignedinto6groupsincludingIcontrolgroupand5operationgroupsinwhichspecimenswerecollectedat1,3,6,9,12postoperativeweeks,respectively.HHKartificialtendonswereimplantedafter
6、tenonectomyintheoperationgroupswhilethecontrolrabbitsreceivedimplantationwithnormalrabbittendon.ThedegradationofHHKartificialtendonwasobservedmo印hologicallyandbyhistochemistryforubiquilindetectionandcytochemistryforacidphosphatae(AcPase).ResultsObservationunderlight
7、microscoperevealedthathumanhaircuticlesbrokeofffromtheimplantedhumanhairandresolved1weekaftertheimplantation,andmacrophagocytesandmultinucleargiantcells(MnGCs)wereseenattachedtothesurfaceofthehumanhairimplant.HHKartificialtendonwasbrokendownintosmallparticles,phagoc
8、ytosedbythemacrophagocytesandMnGCs3to6weeksaftertheimplantation.Enzymecytochemistryforubiquilindetectionproducedstrongpositivereactioninth