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ID:36665510
大小:2.87 MB
页数:47页
时间:2019-05-13
《内蒙古阿尔巴斯白绒山羊毛囊生长周期性变化与KAP6cDNA的特征分析》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、内蒙古农业大学硕士学位论文内蒙古阿尔巴斯白绒山羊毛囊生长周期性变化与KAP6cDNA的特征分析姓名:张春兰申请学位级别:硕士专业:动物遗传育种与繁殖指导教师:李金泉;尹俊2003.5.1摘要本实验以内蒙古阿尔巴斯白绒山羊为研究材料,每月采样一次,研究其绒毛生长周期性变化特点。席0用Sacpic染色法对体侧部皮肤横、纵切片进行染色。由横切切片可见5~8月份次级毛囊逐渐进入兴盛前期,兴盛前期的毛囊是随着旧绒干从毛囊内脱出开始重建的新毛囊;8~12月份为毛囊的兴盛期:1月份大部分进入退行期,2~4月份为休止期。纵切切片表明次级毛囊深
2、度在8月份最深,毛球宽、毛囊宽在3‘月份最宽;退行期的毛囊仍具有活性,绒生长变慢;休止期毛囊失去活性,绒不再生长:兴盛期的毛囊毛球末端扁平,真皮乳头层细胞一(DPCs)内陷;退行期、休止期毛球末端呈尖状,真皮乳头层细胞向外突出√碱长测量结果表明最早于6月份下旬绒开始长出体表,缡惠1;辨最快时期为10月份,并非为最冷时期:绒长出体表即使在同一品种内因个体不同而有差异,同一个体的不同部位也具有不同步性:绒先在股部长‘出,然后是体侧部,最后是肩胛部。选择10月份周岁母羊体侧部皮肤构建兴盛期cDNA质粒文库。随机挑选克隆提取质粒用Mt
3、3正向通用引物对插入片段进行测序,得到636个ESTs序列。与Genebank数据库Blastn比对后发现有41个ESTs与绵羊的角蛋白偶联蛋白KAP6.1基因期望值4、壹助相盼∞eo辞PeriodicvarietyofhairfolliclesaboutInnerMongoliaARBASwhitecashmeregoatsandcharacteristicofKAP6eDNAAbstractThisexperimentregardstheARBASwhitecashmeregoatsinInnerMongoliaasstudymaterials.WeCUtskinsmonthlytostudytheirhairfollicleschangeinayear,makinguseoftheSac5、picdyingmethodtostudyhorizontal,perpendicularslices.FromhorizontalslicesWC俞dthatsecondaryfolliclesfSFs)enterintopro-anagenfromMaytoAugustgradually.Newpro-anagenfolliclesrebuildfromoldfollicles.FromAugusttoDecember,theyenterintoanagen.MostofthementerintoealageninJanu6、ary.TheperiodfromFebruarytoAprilistheirtelagen.TheperpendicularslicesshowthatSFs’depthisthemostdeepinAugust囊7、ds.—_射andthebreadthofbulbsandhairgermsaremostwidein和钾l砖IncatagenSFsstillhaveactivity,butcashmeresgrowslowly.IntelagenSFslosetheiractivityandcashmeresnolonger8、grow.Inanagen,bulbsareflatanddermalpapillacelIs(DPC)sinkinside.IncatagenandtelagenbulbsaresharpandDPCsareoutwardly.ThemeasuringresultshowsthatcashmeresgrowoutskinearliestinJuneandgrowfastinOctoberinayear,butOctoberisnotthecoldestmonth.Thetimeofcashmeresgrowingoutofs9、kinisdifferentindividually,evendifferentateverypartinsameindividual.Cashmeresgrowoutfromhipfirstly,thenfromthesideofthebody,finallyfromsho
4、壹助相盼∞eo辞PeriodicvarietyofhairfolliclesaboutInnerMongoliaARBASwhitecashmeregoatsandcharacteristicofKAP6eDNAAbstractThisexperimentregardstheARBASwhitecashmeregoatsinInnerMongoliaasstudymaterials.WeCUtskinsmonthlytostudytheirhairfollicleschangeinayear,makinguseoftheSac
5、picdyingmethodtostudyhorizontal,perpendicularslices.FromhorizontalslicesWC俞dthatsecondaryfolliclesfSFs)enterintopro-anagenfromMaytoAugustgradually.Newpro-anagenfolliclesrebuildfromoldfollicles.FromAugusttoDecember,theyenterintoanagen.MostofthementerintoealageninJanu
6、ary.TheperiodfromFebruarytoAprilistheirtelagen.TheperpendicularslicesshowthatSFs’depthisthemostdeepinAugust囊
7、ds.—_射andthebreadthofbulbsandhairgermsaremostwidein和钾l砖IncatagenSFsstillhaveactivity,butcashmeresgrowslowly.IntelagenSFslosetheiractivityandcashmeresnolonger
8、grow.Inanagen,bulbsareflatanddermalpapillacelIs(DPC)sinkinside.IncatagenandtelagenbulbsaresharpandDPCsareoutwardly.ThemeasuringresultshowsthatcashmeresgrowoutskinearliestinJuneandgrowfastinOctoberinayear,butOctoberisnotthecoldestmonth.Thetimeofcashmeresgrowingoutofs
9、kinisdifferentindividually,evendifferentateverypartinsameindividual.Cashmeresgrowoutfromhipfirstly,thenfromthesideofthebody,finallyfromsho
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