阻断心肌血管aibp基因表达对心肌微血管再生作用的研究

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1、分类号R54UDC616.1密级公开阻断心肌血管AIBP基因表达对心肌微血管再生作用的研究马晓磊培养类别全日制学位类型专业学位一级学科(专业类)临床医学二级学科(专业)内科学（心血管病）研究方向冠心病基础与临床指导教师吕安林副教授副主任医师培养单位西京医院心内科二O一五年五月第四军医大学硕士学位论文目录缩略语表·······························································································1中文摘要··························

2、·····································································3英文摘要·······························································································6前言······························································································10文献回顾··········

3、····················································································11正文······························································································21第一部分大鼠CMECS体外分离、培养和鉴定及慢病毒转染筛选···················211.材料············································

4、···············································211.1实验动物················································································211.2主要试剂················································································221.3实验仪器················································

5、································222.方法···························································································222.1大鼠CMECs分离与培养····························································222.2大鼠CMECs的鉴定·····························································

6、·····242.3慢病毒转染阻断大鼠CMECsAIBP基因表达··································242.4统计学分析·············································································293.结果···························································································303.1倒置显微镜下CMECs形态学特征···········

7、·····································303.2内皮细胞吞噬Dil-Ac-LDL荧光染色结果·······································303.3荧光显微镜下观察慢病毒转染情况···············································313.4筛选慢病毒转染基因序列的RT-PCR检测结果································313.5筛选慢病毒转染基因序列的WesternBlot检测结果·····················

8、······324.讨论··············