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ID:35000632
大小:1.69 MB
页数:30页
时间:2019-03-15
《创伤性关节炎关节滑液中炎性细胞因子的表达及意义的临床研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、分类号:R68密级:不保密UDC:617.3学校代码:11065硕士学位论文创伤性关节炎关节滑液中炎性细胞因子的表达及意义的临床研究蒋春雨指导教师孙康教授学科专业名称外科学(骨外)论文答辩日期2015年5月31日摘要研究目的:通过检测创伤性关节炎(PTA)(膝关节)TNF-α同IL-1关节液中的浓度,观察两者同PTA的X线分期间的关系。探讨两者得浓度同PTA的病变严重程度之间的内在联系,从而能够寻找出反映PTA病变的程度的生化指标。材料与方法:从2014年1月至2014年6月来院就诊的初诊患者进行性Kellgren&Lawrence分级,然后从中随机抽样选择膝PTA患者40例(每
2、级各10例),其中女患者19人,男患者21人,平均年龄为25.49岁,年龄范围为18-30岁。实验者分为实验组(轻度、重度)2组,每组20名患者,抽取并检测膝关节滑液中的炎性细胞因子TNF-α同IL-1的浓度。再随机抽样选择10名无其他膝关节疾病的健康志愿者作为对照组,其中女患者4人,男患者6人,平均年龄为24.59岁,年龄范围为19-32岁,同样抽取并检测膝关节关节滑液中TNF-α同IL-1的浓度。关节液中TNF-α同IL-1得浓度检测采用ELISA法。应用SPSSl9.0软件进行数据分析,采用t检验观察分析各组间的数据有无统计学意义;采用Pearson相关分析观察分析各组间的
3、浓度数据与骨关节炎影像学X线分级间得关系有无统计学意义。结果:对照组TNF-α同IL-1在关节滑液中的浓度均低于实验组,有统计学差异(P4、介素-1;创伤性关节炎;骨关节炎X线分级;AbstractObjective:PurposeofthestudyistodetecttheconcentrationofTNF-αandIL-1inthesynovialfluidofPTA.ToobservetherelationshipsbetweenbothTNF-α、IL-1andkneepost-traumaticarthritisX-stage(Kellgren&Lawrencecriteria).Toinvestigatetheinternalrelationshipsbetweeninflammatorycytoki5、nesandtheseverityofkneePTA,andtosearchbiochemicalindicatorstoreflectthedegreeofPTA.Materialandmethod:Weselectedrandomly40patients(21malesand19females,18-30age)withkneePTAfromouroutpatientsandinpatientin2014(sinceJanuarytoJune).ThepatientsaccordingtoX-stage(Kellgren&Lawrencecriteria)weredivide6、dinto2groups(lightandheavy),20patientsineachgroup,extracteffusionofkneejoint,measuredTNF-αandIL-1potency.Inadditiontorandomlyselect10healthyvolunteers(nootherkneejointdisease)thesameextractionkneejointfluidinspectionascontrol,Ofwhich6weremale,4female,age19-32yearsold,averageageis24.59yearsold7、l.Adoptenzyme-linkedimmunosorbentassay(ELISA)wasdetectedTNF-αandIL-1.ApplyingSPSSl6.0statisticalanalysissoftware,ttest,theconcentrationofexperimentalobservationofTNF-αandIL-1ineachgrouphasnostatisticaldifference.AndusingPearsoncorrelationanal
4、介素-1;创伤性关节炎;骨关节炎X线分级;AbstractObjective:PurposeofthestudyistodetecttheconcentrationofTNF-αandIL-1inthesynovialfluidofPTA.ToobservetherelationshipsbetweenbothTNF-α、IL-1andkneepost-traumaticarthritisX-stage(Kellgren&Lawrencecriteria).Toinvestigatetheinternalrelationshipsbetweeninflammatorycytoki
5、nesandtheseverityofkneePTA,andtosearchbiochemicalindicatorstoreflectthedegreeofPTA.Materialandmethod:Weselectedrandomly40patients(21malesand19females,18-30age)withkneePTAfromouroutpatientsandinpatientin2014(sinceJanuarytoJune).ThepatientsaccordingtoX-stage(Kellgren&Lawrencecriteria)weredivide
6、dinto2groups(lightandheavy),20patientsineachgroup,extracteffusionofkneejoint,measuredTNF-αandIL-1potency.Inadditiontorandomlyselect10healthyvolunteers(nootherkneejointdisease)thesameextractionkneejointfluidinspectionascontrol,Ofwhich6weremale,4female,age19-32yearsold,averageageis24.59yearsold
7、l.Adoptenzyme-linkedimmunosorbentassay(ELISA)wasdetectedTNF-αandIL-1.ApplyingSPSSl6.0statisticalanalysissoftware,ttest,theconcentrationofexperimentalobservationofTNF-αandIL-1ineachgrouphasnostatisticaldifference.AndusingPearsoncorrelationanal
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