蜂针对佐剂性大鼠关节炎滑膜细胞因子基因表达的影响

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1、蜂针对佐剂性大鼠关节炎滑膜细胞因子基因表达的影响杨顺益林军邓金峰冯淑兰李万瑶张宏刘金芝(广州中医药大学，39蜂疗网，广州510407)内容提要目的:检测IL-1B、IL-6在类风湿性关节炎(RA)的表达，探讨蜂针治疗RA的作用机理。方法:将40只大鼠随机分为四组，采用PCR微板核酸杂交-ELISA方法检测滑膜IL-1B、IL-6的mRNA表达水平。结果:蜂针组和激素组IL-1B、IL-6的mRNA表达水平无显著性差异，而与模型组比较有显著性差异。结论:RA早期IL-1B、IL-6的mRNA表达水平是上调的，蜂针治疗后可以抑制

2、其转录和表达，有利于RA病情的缓解。关键词蜂毒疗法类风湿IL-1BmRNAIL-6mRNA基因表达EffectofAcupoint-apitherapyonCytokineGeneExpressionofSynovialTissuesinAdjuvantArthritisRatsYANGShunyi，LINJun，DENGJinfeng，FENGShulan，LIWanyao，ZHANGHong，LIUJinzhi(GuangzhouUniversityofTCM，Guangzhou，510407)Objective:Tos

3、tudytheeffectofacupoint-apitherapyontheexpressionofIL-1BmRNAandIL-6mRNAandtoanalyzeitsmechanismsintreatmentofrheumatoidarthritis(RA).Methods:Atotalof40ratswererandomlyandevenlydividedintonormalcontrolgroup，modelgroup，hormone(hydrocortisone)groupandacupoint-apithera

4、pygroup.RAmodelwasestablishedbyinjectingFreund.sadjuvant(0.05mL)intotherighthind-paw.Forratsofhormonegroup，intraperitonealinjectionofhydrocortisone(10mg/2mL)wasperformed.Inacupoint-apitherapygroup，/Shenshu0(BL23)，/Zusanli0(ST36)，/Mingmen0(GV4)and/Dazhui0(GV14)weres

5、timulatedwithbee-needleprickingmethod.IL-1BmRNAandIL-6mRNAexpressionofsynovialtissues(rightkneejointcapsule)wasdetectedusingPCRmicroplatesandwichhybridizationtechnique.Results:Incomparisonwithnormalcontrolgroup，IL-1BmRNAandIL-6mRNAexpressionofsynovialtissuesincreas

6、edconsiderablyinmodelgroup(P<0.01).Therewasnostatisticaldifferencebetweenacupoint-apitherapygroupandhormonegroupinIL-1BmRNAandIL-6mRNAexpression，buttheexpressionsinthesetwogroupsweresignificantlyweakerthanthoseofmodelgroup.Conclusion:Acupoint-apitherapycouldinduceu

7、pregulationofIL-1BmRNAandIL-6mRNAexpressioninRArats，whichmayberesponsibleforapitherapygeneratedreliefofsymptomsandsignsofRA.KeyWordsApitherapyRheumatoidarthritisIL-1BmRNAIL-6mRNAGeneexpression类风湿性关节炎(rheumatoidarthritis，RA)是一种以关节滑膜炎为特征的慢性全身性自身免疫性疾病，其病理特征是滑膜慢性炎症。其关节

8、损伤的病理过程与细胞因子网络失调密切相关，其中白细胞介素1(interleukin-1，IL-1)和白细胞介素6(interleukin-6，IL-6)异常表达和失调可能是其发病的重要因素。本文采用PCR微板核酸杂交-ELISA方法[1，2]，观察蜂针对RAIL-1B、IL-6mRNA表达