滤泡辅助性t细胞及白介素-21在类风湿关节炎外周血和关节滑液中的表达及临床意义

《滤泡辅助性t细胞及白介素-21在类风湿关节炎外周血和关节滑液中的表达及临床意义》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、授予单位代码10089学号或申请号13480HebeiMedicalUniversity硕士学位论文在职科学学位滤泡辅助性T细胞及白介素-21在类风湿关节炎外周血和关节滑液中的表达及临床意义作者姓名：孙凤艳导师：郭惠芳教授专业：内科学二级学院：第二医院2015年05月河北医科大学学位论文使用授权及知识产权归属承诺本学位论文在导师（或指导小组）的指导下，由本人独立完成。本学位论文研究所获的研究成果，其知识产权归河北医科大学所有。河北医科大学有权对本学位论文进行交流、公开和使用。凡发表与学位论文相关的论文，第一署名单位为河北医科大学，试验材料、原始数据、申报的专利等知识产权均归河北医科大学所

2、有。否则，承担相应的法律责任。研究生签名洛A‘导师签章年&月>河北医科大学研究生学位论文独创性声明本论文是在导师指导下进行的研究工作及取得的研究成果，除了文中特别加以标注和致谢等内容外，文中不包含其他人己经发表或撰写的研究成果，指导老师对此进行了审定。本论文由本人独立撰写，文责自负。研究生签名^^导师签章〔y年6月）目录中文摘要·············································································1英文摘要··················································

3、···························2英文缩写·············································································4研究论文滤泡辅助性T细胞及白介素-21在类风湿关节炎外周血和关节滑液中的表达及临床意义前言·············································································6材料与方法···························································

4、·········7结果·············································································9附表·············································································11讨论·············································································15结论···········································

5、··································19参考文献·······································································19综述T细胞亚群在类风湿关节炎中的作用·································23致谢···················································································30个人简历········································

6、·····································31中文摘要滤泡辅助性T细胞及白介素-21在类风湿关节炎外周血和关节滑液中的表达及临床意义摘要目的：本实验拟采用流式细胞术（FCM）和酶联免疫吸附（ELISA）的方法分别检测类风湿关节炎（RA）患者外周血单个核细胞（PBMC）和关节液中CD4+CXCR5+T细胞的表达以及相关细胞因子白细胞介素（IL）-21的表达，探讨滤泡辅助性T细胞(Tfh)在类风湿关节炎发病机制中的作用。方法：采集RA病人和对照组（骨关节炎患者）外周血、关节液，流式细胞术检测外周血及关节液中CD4+CXCR5+T细胞的百分比；血清及关节液中IL

7、-21蛋白的水平用酶联免疫吸附法（ELISA法）检测。结果：RA组活动期及缓解期外周血单个核细胞中Tfh细胞百分率（14.15%±2.74%，12.80%±2.15%）均明显高于对照组（7.26%±1.62%）（P＜0.05），活动期与缓解期组间比较，差异无统计学意义；RA组活动期及缓解期IL-21水平（41.88±15.63pg/ml，37.27±11.44pg/ml）均明显高于对照组（22.25±1.18）pg/ml（P＜0.0