资源描述：
《用issr标记分析红麻种质资源的遗传多样性》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。
1、武汉植物学研究2008,26(3):240～244JournalofWuhanBotanicalResearch用ISSR标记分析红麻种质资源的遗传多样性3林荔辉,汪斌,陶爱芬,吴建梅,孙志强,方平平,祁建民(福建农林大学生命科学学院/作物科学学院,福州350002)摘要:为明确红麻种质资源的遗传多样性和亲缘关系,以20个国家的32份红麻(HibiscuscannabinusL.)种质资源为材料,从90个ISSR引物中筛选出22个多态性引物对供试材料进行PCR扩增,最终选用条带最清晰、多态性明显的13个引物的扩
2、增数据进行统计分析。结果表明:(1)13条ISSR多态性引物共扩增出谱带总数59条,多态性条带总数为47条,多态性条带比率达79166%,其遗传多样性较丰富;(2)在切割线L1取值为0173时,可将供试材料分为一个由22份栽培品种构成的L1-1大类群,以及由8份野生材料和2份古老地方品种构成的3个独立的个类和3个不同小类群。(3)当切割线L2取值为0177时,又可将第1个大类群L1-1中22份栽培品种根据其亲缘关系,重新划分为I1、I2、I3、I44个不同的亚类群,各亚类群表现出明显的地域性特点。(4)聚类结果
3、显示,红麻多数栽培品种之间的遗传差异较小,亲缘关系较近,其遗传多样性比野生材料明显狭窄。关键词:红麻;种质资源;ISSR;遗传多样性;聚类分析中图分类号:Q75;S563文献标识码:A文章编号:10002470X(2008)0320240205TheAnalysisofGeneticDiversityofKenafGermplasmBasedonInter2simpleSequenceRepeats3LINLi2Hui,WANGBin,TAOAi2Fen,WUJian2Mei,SUNZhi2Qiang,FANG
4、Ping2Ping,QIJian2Min(CollegeofLifeorCropScience,FujianAgricultureandForestryUniversity,Fuzhou350002,China)Abstract:Inordertoprobetherelationshipandgeneticdiversityofthekenaf(Hibiscuscannabinus)germplasm,32kenafvarietiesfrom20countrieswerestudied,and22primers
5、screenedfrom90ISSRprimerswereusedtoamplifytheDNA,fromwhich13primerswithpolymorphicandclearbandswereanalysized.Theresultswereasfollows:(1)59bandswereamplifiedbasedon13primers,amongwhich47bandswerepolymorphic,andthepercentageofpolymorphicbandswas79166%,whichin
6、dicatedthegeneticdiversitywascorrespondinglyrich.(2)The32kenafaccessionscouldbeclassifiedintotwogroupsatthelevelofD=0173:onelargegroupnamedL1-1constitutedof22cultivars,thereantiquekindsandtheresubgroupincluding8wildvarietiesand2antiquelocalspecies.(3)The22cu
7、ltivarsinthefirstgroupL1-1couldbedividedinto4subclasses(I1,I2,I3,I4)atthelevelofD=0177,whichhadvisibleregionalcharacter.(4)Theclusterresultindicatedthatmostofthecultivarshadsmallgeneticdiversityandrelationship.Itwasobviousthatthebasisoftheinheritanceofkenafc
8、ultivarswerecomparativelynar2rowerthanthewildspecies.Keywords:Kenaf(HibiscuscannabinusL.);Germplasm;ISSR;Geneticdiversity;Clusteranalysis[2]红麻(HibiscuscannabinusL.,kenaf)为锦葵科木水平。随着生物技术的发展,进一步深化红
