资源描述：
《A genomic variation map provides insights into the genetic.pdf》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、lettersAgenomicvariationmapprovidesinsightsintothegeneticbasisofcucumberdomesticationanddiversityJianjianQi1,12,XinLiu2,12,DiShen1,12,HanMiao1,12,BingyanXie1,12,XixiangLi1,12,PengZeng2,ShenhaoWang1,YiShang1,XingfangGu1,YongchenDu1,YingLi1,TaoLin1,JinhongYuan1,Xu
2、eyongYang1,JinfengChen3,HuimingChen4,XingyaoXiong1,5,KeHuang5,ZhangjunFei6,LinyongMao6,LiTian7,ThomasStädler8,SusanneSRenner9,SophienKamoun10,WilliamJLucas11,ZhonghuaZhang1&SanwenHuang1Mostfruitsinourdailydietaretheproductsofdomesticationcucumbergenomeatsingle-b
3、aseresolutionbyperformingdeeprese-andbreeding.Herewereportamapofgenomevariationforaquencingofall115lines,andwealsosequencedthewildcucumbermajorfruitthatencompasses~3.6millionvariants,generatedbygenomedenovoandcomparedittothegenomeofcultivatedcucum-deepresequenci
4、ngof115cucumberlinessampledfrom3,342ber13.Thesegenomicresourcesgeneratenewinsightsintothegeneticaccessionsworldwide.Comparativeanalysissuggeststhatfruitbasisofdomesticationanddiversityforthisimportantcrop.cropsunderwentnarrowerbottlenecksduringdomesticationReseq
5、uencingofthe115linesgeneratedatotalof7.275billionthangraincrops.Weidentified112putativedomesticationpaired-endreads(632Gbofsequence),withaveragedepthof18.3×andsweeps;1oftheseregionscontainsageneinvolvedinthelossofcoverageof95.2%(SupplementaryTable1).Throughcompa
6、risonwithbitternessinfruits,anessentialdomesticationtraitofcucumber.thereferencegenomeoftheinbredcucumberline9930(refs.13,14),Wealsoinvestigatedthegenomicbasisofdivergenceamongwedetectedatotalof3,305,010SNPs,336,081smallinsertionsandthecultivatedpopulationsanddi
7、scoveredanaturalgeneticdeletions(indels;shorterthan5bp)and594presence-absencevari-variantinab-carotenehydroxylasegenethatcouldbeusedtoations(PAVs)(Table1,SupplementaryFigs.1–4,Supplementarybreedcucumberswithenhancednutritionalvalue.ThegenomicTables2–4,Supplement
8、aryNoteandSupplementaryDataSet).Wehistoryofcucumberevolutionuncoveredhereprovidesthealsocarriedoutdenovosequencingandassemblyofthewildcucumberbasisforfuturegenomics-e
