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1、ComparativeTranscriptionalProfilingProvidesInsightsintotheEvolutionandDevelopmentoftheZygomorphicFlowerofViciasativa(Papilionoideae)ZhipengLiu*,LichaoMa,ZhibiaoNan,YanrongWang*StateKeyLaboratoryofGrasslandAgro-ecosystems,SchoolofPastoralAgriculturalScienceandTechnology,LanzhouUniversity,
2、Lanzhou,ChinaAbstractBackground:Viciasativa(thecommonvetch)possessesapredominantzygomorphicflowerandbelongstothesubfamilyPapilionoideae,whichisrelatedtoArabidopsisthalianaintheeurosidIIcladeofthecoreeudicots.Eachvetchflowerconsistsof21concentricallyarrangedorgans:theoutermostfivesepals,t
3、henfivepetalsandtenstamens,andasinglecarpelinthecenter.Methodology/PrincipalFindings:Weexploredthefloraltranscriptometoexamineagenome-scalegeneticmodelofthezygomorphicflowerofvetch.mRNAwasobtainedfromanequalmixtureofsixfloralorgans,leavesandroots.Denovoassemblyofthevetchtranscriptomeusin
4、gIlluminapaired-endtechnologyproduced71,553unigeneswithanaveragelengthof511bp.Wethencomparedtheexpressionchangesinthe71,553unigenesintheeightindependentorgansthroughRNA-SeqQuantificationanalysis.Wepredominantlyanalyzedgeneexpressionpatternsspecifictoeachfloralorganandcombinationsoffloral
5、organsthatcorrespondedtothetraditionalABCmodeldomains.ComparativeanalyseswereperformedinthefloraltranscriptomesofvetchandArabidopsis,andgenomesofvetchandMedicagotruncatula.Conclusions/Significance:OurcomparativeanalysisofvetchandArabidopsisshowedthatthevetchflowersconformtoastrictABCmode
6、l.WeanalyzedtheevolutionandexpressionoftheTCPgenefamilyinvetchatawhole-genomelevel,andseveralunigenesspecifictothreedifferentvetchpetals,whichmightoffersomecluestowardelucidatingthemolecularmechanismsunderlyingfloralzygomorphy.Ourresultsprovidethefirstinsightsintothegenome-scalemolecular
7、regulatorynetworkthatcontrolstheevolutionanddevelopmentofthezygomorphicflowerinPapilionoideae.Citation:LiuZ,MaL,NanZ,WangY(2013)ComparativeTranscriptionalProfilingProvidesInsightsintotheEvolutionandDevelopmentoftheZygomorphicFlowerofViciasativa(Papilionoideae).PLoSONE8(2)
