供精人工授精不同结局精子蛋白质组成差异的研究

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1、硕士学位论文学校代码：10023学号：$2010020008供精人工授精不同结局精子蛋白质组成差异的研究Proteomeanalysisofspermindifferentoutcomes^o，'‘‘1·1orartltlClaJ-1nsemlnatlonbydonor所院：国家人口计生委科学技术研究所姓名：李志广指导教师：谷翊群研究员导师小组：于和鸣卢文红辛玲学科专业：外科学研究方向：泌尿男科完成日期：2013年4月20日北京协和医学院硕士研究生学位论文唧幽舢舢舢删删舢舢删Y2340232目录中文摘要································

2、···．·．．···．．．．．．．．．．．．．．．．．．．1英文摘要··········--·····················-·····-·····················2—1上．—j一日H舌”一“”一”一””‘。。‘。。··。。-····--··-·······················3第一部分：供精人工授精不同结局精子蛋白质组成差异实验材料·························---·-·········0Q411················8实验方法·······-·························-

3、·······················10实验结果·······-·················································14实验讨论······-··················-············-········-·········16实验小结·································-··················-····18参考文献·-····················--··-·························-····19第二部分：供精人工

4、授精不同结局精子差异蛋白质谱鉴定实验材料-···················-············-············-··········21实验方法················································-·-······21实验结果··········-·-·---·································-·-····23实验讨论··········································-·····-········25实验小结··············

5、············--··-··························29参考文献·-·······················································30第三部分：精液凝固蛋白．1和谷胱甘肽硫转移酶Mu3蛋白在供精人工授精不同结局精子蛋白中的表达差异实验材料·············-·-··············-··························33实验方法······································-·············-····35实验结果

6、················-················-·······················37实验讨论·························································37实验小结····-·············---······-······-··················-···39参考文献·························································40结论············-·········--············

7、-······-·················-···42文献综述··-·····-····················································43英文缩略词表(Abbreviation)······-···············-···················52致谢···············-······································-······53个人简历·········-·······················-···--··············