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ID:34161234
大小:7.98 MB
页数:48页
时间:2019-03-04
《小檗碱和阿霉素联合使用药理作用的分析》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、ABSTRACTThestudies.ofpharmacologiceffectofcombinedherberineWltnd0X0rUDicin●l_●●J■l■PharmaceuticalanalysisPostgraduateTongNannanTutorZhaoXiaoyanDoxorubicinisaveryeffectiveandoftenusedanti-tumordrug.Butitsseriousdose-dependentsideeffectslargelylimitedtheclinicaluseage.Theaimofthisstudyistoinvestig
2、atewhetherberberinecouldprotectagainstdoxorubicin—inducedcardiotoxicityandliverinjuryinmice,andtoinvestigatewhethercombinateberberinewimdoxombicinwouldgeneratesynergisticanticancereffect.Inanimalstudy,fortyhealthymicewererandomlydivitedintofourgroups:controlgroups;doxorubicin-treatedgroups;berbe
3、rine+doxorubicin-treatedgroups;berberine—treatedgroups.Themiceweretreatedwithdrugseverytwodays,for7doses.Inthefourteenthdays,bodyweightandECGchangeswereobserved.Attheendofthestudy,bloodwasdrawnfromtheinnercanthus,theactivityofLDH、ALTandASTintheserumweretested.HEstainingforhistopathologicalanalys
4、isintheheartandliverwereperformedunderthemicroscope.TheresultshowedthatasignificantdeclineinbodyweightWasobservedindoxorubicin—treatedgroups.butdoxorubicin-induceddeclineinbodyweightWasreducedbypretreatedwithberberine.Therewasnochangeintheweightofberberine-treatedmic.e.Comparedwithcontrolgroup,p
5、rolongationofQRSdurationandincreasedplasmaLDH、ALTandASTactivitieswereobservedindoxorubicin.treatedmice,andthesechangesweresignificantlypreventedbypretreatmentwithberberine.Histopathologicalstudiesshowedthatdoxorubicincausedstructuralinjuries,suchasvascularcongestion,inflammatorycellinfiltration,
6、cellnecrosisandfibrosisintheheartandliver.ThesehistopathologicalchangeswereIII西南大学硕士学位论文largelyattenuatedbyberberinepretreatment,andberberinecausednohistopathologicalchangeinberberine-treatedgroups.Allthesefindingsindicatedthatberberinehadthecardioprotectiveandhepatoptotecfiveondoxorubicin-induc
7、edheartandliverinjuryinmice.Inthisstudy,The3-(4,5-dimethyl一2·thiazolyl)-2,5-diphenyl·2-tetrazoliumbromide(MTT)assaywasusedtodetectthecellviabilityofA549,HeLaandHepG2cellsaftereachcelllinewastreatedwimDOX.BERoracombinationofD
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