桑籽降血糖物质提取纯化工艺及药理活性初步的研究

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1、ResearchonExtractionandPurificationandpreIiminarypharmacologicalActivityofhypoglycemicSubstancefromMulberrySeedSpeciaIity:PharmacyofTraditionalChineseMedicineAuthor:LvZhiqiangTutor:ProfessorTIANJing-zhenAbstractWehaveresearchedonextraction,PurificationandPharmacologicalactivityaboutthistechnolog

2、yforsystemtest.Finally,wedeterminedtheprocessforindustrialproductionofMulberrySeedextractionandPurificationandPharmacologicalactivity.FirstlywehaveestablishedarapidandsimplemethodofthecrudeextractofFat，totalalkaloids(DNJ),totalflavone，totalpolysaccharides,compareddifferentextractionmethodsanddet

3、erminedtheprocessforindustrialproductionofsteiosideextractionandisolation.Onthisbasis,throughmacroporousresin-polyamideresinusedincombination,wehavedeterminedthetotalflavonehasgoodadsorptionresinanditsseparationprocess.Furthermore,throughalcoholprecipitationandremovingprotein,dialysis,wehavedete

4、rminedthetotalpolysaccharideenrichmenttechnology.Next,throughtheremovalofprotein,50%alcoholprecipitationimpurity,n-butanol,wedeterminedthealkaloidenrichmenttechnology.Finally,throughasingleeffectivepartandeffectivepartsofcompatibilityCompatibility,Wehavedeterminedthemosteffectivehypoglycemiccomp

5、onent.TheresultsshowthatMulberryseedinthesitealreadyextractionprocessofthetotalflavonoidsextraction,purityofabout52.3%,totalpolysaccharideswas64.4%,thepurityof35.6%totalalkaloids，pharmacologicalvalidation,alkaloidsandpolysaccharideshaveexcellenthypoglycemicactivity,Thistechnologyprovidessomeevid

6、enceformulberryseedcapsuledevelopmentandhealthfooddevelopment.Keywords:Mulberryseed;extraction;purification;pharmacologicalactivity;technology重点词汇英文缩写糖尿病DiabetesmellitusDM1-脱氧野尻霉素1-deoxynojirimycin1-DNJ总多糖TotalmuchpolysaccharidesTMP苑代甲氧酰氯Fluorenemethoxyofchloride-ClFM0C-C1四氧卩密淀AlloxanA

7、LX第一部分课题研究思路第二部分桑籽油提取工艺研究1仪器与材料1.1仪器1.2材料2实验方法：2含油率测定：4.结果与分析：4.1不同提取方法的对桑籽油提取率的影响4.2溶剂对桑籽油提取率的影响：4.3提取时间对桑籽油提取率的影响：4.4溶剂倍量对桑籽油提取率的影响4.5提取温度对桑籽油提取率的影响4.6桑籽油提取的正交试验4.6.1桑籽油提取的正交试验结果4.6.2正交试验的方差分析结果4.6.3优选提取