黄体酮对大鼠脑缺血再灌注后血脑屏障通透性影响与其机制的分析

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1、din的破坏，对BBB用的机制之一。关键词黄体酮闭锁小带蛋白．1咬合蛋白脑缺血再灌注血脑屏障TheFifthClinicalCollegeZhengzhou，Henan，450052，ChinaAbstractBackgroudand0bjectiveincidenceofcerebrovasculardisease(CVD)iSincreasingwithmoreandmoreagingpopulation．CVDhasbecameoneofthethreeleadingcausesofdeathinmankind．Itshighincidence，

2、disabilityandmortalityratesbringheavyeconomicandmentalburdentosocietyandfamily．CVDisdividedintotwotypes：ischemicandhemorrhagic．Ischemiccerebrovasculardiseaseaccountsforabout80％．Currently,theeffectivetreatmentofacuteischemicstrokeisthrombolytictherapyintimewindow．Itbringshopetopa

3、tientswithcerebralinfarction，butalsobringstheproblemofreperfusioninjury．Cerebralischemia—reperfusioninjuryhascomplexpathophysiologicalprocess，andthedestructionofblood-brainbarrierisoneimportantfactor．Blood．brainbarrier(BBB)，whichplaysanimportantroleintheformationofvasogenicbrain

4、edema，isanimportantguaranteetostableofinternalenvironmentofthecentralnervoussystem．ThebasicstructureofBBBincludesbrainmicrovascularendothelialcells(BMECs)andtightjunction(TJ)，basementmembrane，glialmembraneandextracellularmatrixformedbyendfeetofglialcells．TheexpressionofTJprotein

5、sisrelatedcloselytoBBBpermeabilityandbrainedema．ZonulaVandMCA02h／R72hgroups．Progesterone—treatedgroupvehiclegroupweredoneatthepointofthepeakofBBBpermeability．Thereare12ratsineachgroup．Progesterone-treatedgroupvehiclegrouprespectivelyreceivedanintraperitonealinjectionofprogestero

6、ne(8mg／kg)orequalvolumeofvehicleatlhbeforesurgerylh，6hafterreperfusion．2．DetectionofBBBpermeabilityAsamarkerofBBBpermeability，Evansblue(EB)extravasationinthebraintissuewassurveyedbythemeanofspectrophotometrytoevaluatetheBBBpermeability．3．HEstainImmunohistochemistryanalysisHEstai

7、nwasappliedtojudgetheextentofbraindamageischemicregion·SPfstreptavidin．peroxidase)methodwasusedtomeasuretheexpressionVIpermeabilitybegantoincrease，reachedthepeakat24hofreperfusionandwasstillelevatedat72h．Progesteronegroupandvehiclegroupweredoneat24hofreperfusionaftercerebralisch

8、emiafor2h．TheresultshowedthattheamountofEBofpro