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ID:32913481
大小:3.24 MB
页数:47页
时间:2019-02-17
《解毒益气活血方对人脐静脉内皮细胞血管生成及stat3信号通路干预研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、学位论文指导教师姓名:翌夏垫塾援申请学位级别:照专业名称:生医内型兰论文提交时间:垄Q至2生垒旦论文答辩时间:垄Q兰垄生量旦二。一二年四月【目的】中文摘要探讨解毒益气活方法对人脐静脉内皮细胞血管生成及干预STAT3信号通路和MMP.2的研究。【方法】体外培养HUVEC,给予不同浓度的解毒益气活血方药动物血清对HUVEC作用后,观察内皮细胞体外管道形成实验,并拍照观察血管生成抑制的情况;用免疫组化法检测STAT3在细胞中的表达及用免疫组化法检测培养细胞中MMP.2表达。并探讨解毒益气活血方对人脐静脉内皮细胞血管生成的影响,及对STAT3和M
2、MP.2在HUVEC表达的影响。【结果】1、对HUVEC血管形成能力的影响,各用药组HUVEC成管指数明显低于空白对照组(P3、方药动物血清中、高剂量组显著降低(P4、,且与给药浓度呈正相关。因此解毒益气活血方可以通过肿瘤血管生成来防止肿瘤的复发和转移。2、STAT3在HUVEC中的表达与解毒益气活血方药动物血清浓度有关,且与给药浓度呈负相关。3、MMP.2在HUVEC中的表达与解毒益气活血方药动物血清浓度有关,且与给药浓度呈负相关。【关键词】解毒益气活血方,血管生成,STAT3,MMP一2,浓度。ObjectiveAbstractStudyondetoxificationmethodsofQi—liveonhumanumbilicalveinendothelialcellsonangiogenesis5、andstudyoninterventionofSTAT3signaltransductionandMMP一2.MothodsHUVECculturedinvitro,differentconcentrationsofapoisonafterexposuretoanimalseraofYiqihuoxuerecipeonHUVEC,observationofendothelialcelltubeformationexperiments,takepicturesandobservationofangiogenesisinhibitionus6、ingimmunohistochemicaldeceetionofSTAT3expressionincellsbyimmunohistochemicalmethodanddetectionofMMP一2expressioninculturedcells.DetoxicationofsupplementingQiandactivatingbloodcirculationanddiscussiononeffectofhumanumbilicalveinendothelialcellsonangiogenesis,andeffectsonexp7、ressionofSTAT3inHUVECandN【N口.2.Results1、AngiogenesiseffectofHUVEC,medicationHUVECtubeindexsignificantlybelowtheblankcontrolgroup(P<0.05orP<0.01),especiallyinthedetoxicationofYiqihuoxueprescriptioninserulllofanimals,highdosegroupclearly.DetoxieationofYiqihuoxueprescription8、inserumofanimals,highdosegroupcomparedwiththe5-FUGroupalsohassignificantdifferences(P
3、方药动物血清中、高剂量组显著降低(P4、,且与给药浓度呈正相关。因此解毒益气活血方可以通过肿瘤血管生成来防止肿瘤的复发和转移。2、STAT3在HUVEC中的表达与解毒益气活血方药动物血清浓度有关,且与给药浓度呈负相关。3、MMP.2在HUVEC中的表达与解毒益气活血方药动物血清浓度有关,且与给药浓度呈负相关。【关键词】解毒益气活血方,血管生成,STAT3,MMP一2,浓度。ObjectiveAbstractStudyondetoxificationmethodsofQi—liveonhumanumbilicalveinendothelialcellsonangiogenesis5、andstudyoninterventionofSTAT3signaltransductionandMMP一2.MothodsHUVECculturedinvitro,differentconcentrationsofapoisonafterexposuretoanimalseraofYiqihuoxuerecipeonHUVEC,observationofendothelialcelltubeformationexperiments,takepicturesandobservationofangiogenesisinhibitionus6、ingimmunohistochemicaldeceetionofSTAT3expressionincellsbyimmunohistochemicalmethodanddetectionofMMP一2expressioninculturedcells.DetoxicationofsupplementingQiandactivatingbloodcirculationanddiscussiononeffectofhumanumbilicalveinendothelialcellsonangiogenesis,andeffectsonexp7、ressionofSTAT3inHUVECandN【N口.2.Results1、AngiogenesiseffectofHUVEC,medicationHUVECtubeindexsignificantlybelowtheblankcontrolgroup(P<0.05orP<0.01),especiallyinthedetoxicationofYiqihuoxueprescriptioninserulllofanimals,highdosegroupclearly.DetoxieationofYiqihuoxueprescription8、inserumofanimals,highdosegroupcomparedwiththe5-FUGroupalsohassignificantdifferences(P
4、,且与给药浓度呈正相关。因此解毒益气活血方可以通过肿瘤血管生成来防止肿瘤的复发和转移。2、STAT3在HUVEC中的表达与解毒益气活血方药动物血清浓度有关,且与给药浓度呈负相关。3、MMP.2在HUVEC中的表达与解毒益气活血方药动物血清浓度有关,且与给药浓度呈负相关。【关键词】解毒益气活血方,血管生成,STAT3,MMP一2,浓度。ObjectiveAbstractStudyondetoxificationmethodsofQi—liveonhumanumbilicalveinendothelialcellsonangiogenesis
5、andstudyoninterventionofSTAT3signaltransductionandMMP一2.MothodsHUVECculturedinvitro,differentconcentrationsofapoisonafterexposuretoanimalseraofYiqihuoxuerecipeonHUVEC,observationofendothelialcelltubeformationexperiments,takepicturesandobservationofangiogenesisinhibitionus
6、ingimmunohistochemicaldeceetionofSTAT3expressionincellsbyimmunohistochemicalmethodanddetectionofMMP一2expressioninculturedcells.DetoxicationofsupplementingQiandactivatingbloodcirculationanddiscussiononeffectofhumanumbilicalveinendothelialcellsonangiogenesis,andeffectsonexp
7、ressionofSTAT3inHUVECandN【N口.2.Results1、AngiogenesiseffectofHUVEC,medicationHUVECtubeindexsignificantlybelowtheblankcontrolgroup(P<0.05orP<0.01),especiallyinthedetoxicationofYiqihuoxueprescriptioninserulllofanimals,highdosegroupclearly.DetoxieationofYiqihuoxueprescription
8、inserumofanimals,highdosegroupcomparedwiththe5-FUGroupalsohassignificantdifferences(P
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