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1、作物学报ACTAAGRONOMICASINICA2009,35(3):467−474http://www.chinacrops.org/zwxb/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2009.00467野生稻与亚洲栽培稻的遗传多样性1,2232,*段世华李绍清李绍波朱英国123井冈山大学生命科学学院,江西吉安343009;武汉大学植物发育生物学教育部重点实验室,湖北武汉430072;南昌大学
2、生命科学学院,江西南昌330031摘要:为评价野生稻与亚洲栽培稻的遗传多样性及其变异关系,56对SSR引物被用于研究广泛地理分布的55份普通野生稻(其中32份O.rufipogon和23份O.nivara)和25份亚洲栽培稻(14份indica和11份japonica)样本。298个多态性位点被检出,占总扩增等位点的98.68%。野生稻多态性位点的百分比(平均达91%)及Nei’s遗传多样性值(h)明显高于亚洲栽培稻,表明普通野生稻比亚洲栽培稻具更丰富的遗传多样性。UPGMA聚类分析显示野生稻的两个类群(O.
3、rufipogon和O.nivara)关系密切,但在遗传上存在明显的分化,支持其作为两个独立物种的分类观点。许多普通野生稻中籼粳分化尽管不很明显,然而亚洲栽培稻的籼粳亚种分化是明显的。亚洲栽培稻与多年生普通野生稻(O.rufipogon)关系更为密切,符合异源起源的遗传分化模式。关键词:SSR;亚洲栽培稻;普通野生稻;遗传多样性GeneticDiversityofWildRiceandCultivatedRice1,2232,*DUANShi-Hua,LIShao-Qing,LIShao-Bo,andZHUY
4、ing-Guo12CollegeofLifeSciences,JinggangshanUniversity,Ji’an343009,China;KeyLaboratoryofMinistryofEducationforPlantDevelopmentalBio-3logy,WuhanUniversity,Wuhan430072,China;CollegeofLifeSciences,NanchangUniversity,Nanchang330031,ChinaAbstract:Toevaluatethegen
5、eticdiversityandvariationofcommonwildriceandAsiancultivatedrice,55accessionsofcom-monwildrice(32accessionsofO.rufipogonand23accessionsofO.nivara)and25cultivarsofAsiancultivatedrice(14indicaand11japonica)collectedworldwidewereanalyzedby56SSRprimerpairs.Thepo
6、lymorphiclocipercentagewas98.68%and298polymorphiclociweredetected.AcomparisonofthepolymorphiclocipercentageandNei’sgeneticdiversity(h)indicatedthatthegeneticdiversityofcommonwildricewasobviouslyhigherthanthatofAsiancultivatedrice.TheUPGMAclusteranaly-sisrev
7、ealedthattherewasacloserelationshipbetweenO.rufipogonandO.nivara,butdistinctgeneticdifferentiationwasde-tectedbetweenthem,supportingthepiontofviewthattheyaretwoindependentspecies.Theresultsindicatedthatthein-dica-japonicadifferentiationwasnotveryapparentinm
8、anywildricewhereasitwasobviousinAsiancultivatedrice.Accordingtotheclusteranalysis,therewasacloserconsanguineousrelationshipbetweenAsiancultivatedriceandO.rufipogon,whichsupportedthediphyleticoriginhypo
