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1、网络出版时间：2014-03-2616:07网络出版地址：http://www.cnki.net/kcms/doi/10.11844/cjcb.2014.04.0345.html中国细胞生物学学报ChineseJournalofCellBiology2013,36(4):DOI:10.11844/cjcb.2014.04.0345过表达IL-12调节单核系肿瘤细胞向巨噬细胞分化马婷婷熊海玉王秦成凤李紫薇吴碧涛林艳涂植光*(重庆医科大学检验医学院,临床检验诊断学教育部重点实验室,重庆400016)摘
2、要构建过表达白细胞介素-12(interleukin-12,IL-12)的THP-1单核系肿瘤细胞模型,从形态学、巨噬细胞表面标志表达水平及吞噬功能3方面探究过表达IL-12能否调节单核系肿瘤细胞向巨噬细胞分化。结果发现,与空白和空载组相比,IL-12组的THP-1细胞界限不明显,散在细胞增多;呈圆形或不规则圆形,偶见毛刺状突起;核仁模糊或消失,核染色质条索状浓集;CD68mRNA和蛋白表达量均升高(P<0.05)。与空白组相比,CD11bmRNA和蛋白表达量升高不明显(P>0.05),但72hC
3、D11b蛋白表达量明显高于相同培养条件下的空载组(P<0.05)。培养72h后各组细胞吞噬能力比较,IL-12组吞噬率(36±1.2)%高于空白组(15±3.1)%和空载组(28±1.5)%,差异有统计学意义(P<0.05)。上述结果提示,过表达IL-12可以促进单核系肿瘤细胞向巨噬细胞分化。关键词白细胞介素-12(IL-12);过表达;单核系肿瘤细胞;巨噬细胞;分化IL-12OverexpressionRegulatetheDifferentiationofMonocyticTumorCellt
4、oMacrophagocyteMaTingting,XiongHaiyu,WangQin,ChengFeng,LiZiwei,WuBitao,LinYan,TuZhiguang*(CollegeofLaboratoryMedicine,KeyLaboratoryofLaboratoryMedicalDiagnosticsofEducationMinistry,_x±sChongqingMedicalUniversity,Chongqing400016,China)AbstractInthisstu
5、dy,whetherIL-12overexpressioncouldregulatedifferentiationofmonocytictumorcelltomacrophagocytewasinvestigated.Firstly,amonocytictumorTHP-1cellmodeloverexpressingIL-12wasestablished.Furthermore,thechangeofcellmorphology,maturedmacrophagocytemarkerexpres
6、sionsandphagocytosisweredetected.Morphologically,theexperimentalgroupcellswereroundorirregularroundshape,andsomecellmembraneprocesseswereobserved;cellnucleolusbecamefuzzyorcompletelydisappeared;thenuclearchromatinsappeareddenseandcordlike.Comparedwith
7、thecontrolgroupandemptyvectorgroup,itwasfoundthatCD68mRNAandproteinexpressionswereup-regulated(P<0.05).However,comparedwithblankgroup,CD11bmRNAandproteinexpressionshadnoremarkablechangeintheIL-12overexpressiongroup(P>0.05),whereasCD11bproteinexpressio
8、ninexperimentalgroupwassignificantlyhigherthanthatinemptyvectorgroup(P<0.05).Moreover,phagocytosisoftheexperimentalgroupwassignificantlybetterthanthecontrolgroupandemptyvectorgroup(P<0.05).Inconclusion,IL-12overexpressioncouldpromotethediffere
