瘦素通过抑制PPARγ的表达抑制RAW264．7巨噬细胞向破骨细胞分化.pdf

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1、细胞与分子免疫学杂志(ChinJCellMolImmuno1)2015，31(2145·论著·文章编号：1007—8738(2015)02—0145—04瘦素通过抑制PPAR7的表达抑制RAW264．7巨噬细胞向破骨细胞分化王正宇，朱云，杨小中，杨刚，朱卫文，马涛，张健(重庆医科大学附属第一医院骨科，重庆400016)[摘要】目的探讨瘦素对可溶性核因子KB配体的受体活化因子(sRANKL)诱导RAW264．7巨噬细胞向破骨细胞分化的影响及其机制。方法抗酒石酸酸性磷酸酶(TRAP)染色检测瘦素对sRANKL诱导RAW264．7向破骨细胞分化的形态学影响；实时

2、荧光定量PCR检测TRAP及过氧化物酶体增殖物激活受体(PPAR)的mRNA表达；Westernblot法检测TRAP及PPAR~的蛋白表达。结果瘦素和sRANKL联合处理组与单独sRANKL组相比，TRAP染色破骨细胞数目明显减少；TRAP的mRNA和蛋白表达量明显降低；PPAR~的mRNA和蛋白表达量明显降低，且与瘦素浓度呈梯度相关。结论瘦素可能通过抑制PPAR的表达来抑制RAW264．7细胞向破骨细胞分化。[关键词]瘦素；抗酒石酸酸性磷酸酶；过氧化物酶体增殖物激活受体；RAW264．7细胞；破骨细胞分化[中图分类号]R392．11，Q254，R392

3、-33[文献标志码]ALeptininhibitsthediferentiationofRAW264．7macrophagesintoosteoclastsv／adepressingtheexpressionofPPAR7WANGZhengyu，ZHUYun，YANGXiaozhong，YANGGang，ZHUWeiwen，MATao，ZHANGJianDepartmentofOrthopedics，FirstAfiliatedHospital，ChongqingMedicalUniversity，Chongqing400016，ChinalAbstrac

4、tJoHectiveToinvestigatetheefectsofleptinonthediferentiationofRAW264．7cellsintoosteoclastsinducedbysolublereceptoractivatorofnuclearfactor-KBligand(sRANKL)anditspossiblemechanism．MethodsTheefectsofleptinonthediferentiationfr0mosteoclastprecursorcellsintoosteoclastsweredetectedbytar

5、trate-resistantacidphosphatase(TRAP)staining．ThemRNAexpressionsofTRAPandperoxisomeproliferator-activatedreceptory(PPARy)inosteoclastprecursorcelsweremeasuredbyreal-timequantitativePCR(qRT-PCR)．TheproteinexpressionsofTRAPandPPARyweredetectedbyWesternblotting．ResultsComparedwithsRAN

6、KLtreatmentgroup．thediferentiationfrOmosteoclastprecursorcellsintoosteoclastswassignificantlyinhibitedinsRANKLcombinedwithle}ptintreatmentgroups．Addifionally，themRNAandproteinexpressionsofbothTRAPandPPARysignificantlydecreasedinsRANKLcombinedwithIE}ptintreatmentgroupscomparedwitht

7、hesRANKLtreatmentalone．Furthermore，theexpressionofPPARywasreducedinadose-dependentmanner．ConclusionLeptincouldinhibitthediferentiationofRAW264．7cellsintoosteoclastsviainhibitingtheexpressionofPPARy．[Keywords]Ieplin；tartrate-resistantacidphosphatase；peroxisomeproliferator-activated

8、receptor；RAW264．7cels；diferentiat