lncrna调控nfκb信号通路的活化在肝静脉闭塞病中的作用

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1、徐州医学院硕士学位论文TheroleofincRNAinhepaticveno-occlusivediseasebyregulatingtheactivationofNF—KBsignalpathwayAbstractobjectiveToexploretheroleofNF一1出signalpathwayintheprocessofHVODafterallo-geneiebonemarrowtransplantationinmice，andtheactivationofitregulatedbylongnon·codingRNA(I

2、ncRNA)．MethodsMalemice(H-20)aged8-10weeksweredividedintothreegroupsaftertotalbodyirradiation(TBI)，includingTBIgroup，allogeneicbonemarrowtransplantationgroup(BMTgroup)andbonemarrowcombinedwithendothelialprogenitorcellstransplantationgroup(EPCgroup)，normalmicewereneededas

3、contr01．Liverfunctionwasdetectedonday7，15，33aftertransplantation，histologicalchangeswereevaluatedbylightmicroscopy．Thenhepatocytewereseparated，afterthattotalRNAandtotalproteininhepatocytewereextracted，afterthatthechangesofmRNAandproteinlevelsofmoleculesinNF—rd3signalpat

4、hwaywhichinvolveinhepatocyterepairandlivefibrosisweredetectedbyquantitativePCRandWesternblot．TNF—o【andIL-6levelinserumweremeasured．Thenanalyzetheresultsofmicroarrays．screenlncRNAsassociatedwithNF—r_Bsignal，andconformthefunctionsoftheseslncRNAs．Results1．Liverfunctionhavs

5、beenimprovedinbothBMTgroupandEPCgroup：thelevelsofALT，ASTandbilirubininserumdecreasedcontinuouslyaftertransplantationintheBMTgroup，thebilirubinlevelintheEPCgrouphadsimilarchangewiththeBMTgroup，thelevelofASTdecreasedandthelevelofALTincreasedintheEPCgroup，comparedwiththeBM

6、Tgroup，thelevelsALT，ASTandbilimbindecreasedintheEPCgroup(P

7、juryofhepatocytehasbeenalleviatedintheEPCgroupandliverfibrosisappearedintwogroups．Onday33，therepairofinjuriedlivercellshasbeenrepaired，butliverfibrosisaggravatedinthetwogroups．Onday15，theexpressionofcyclinDlandcyclinElincreasedintheEPCgroupcomparedwiththeBMTgroup(P

8、)，theexpressionofCCL2，CXCL2，CXCR4andTGFflldecreasedintheEPCgroup(P<0．05)onday33．2．4徐州医学院硕士学位论文TheactivationofN