ctgf和hif1α在慢性马兜铃酸肾病大鼠肾小管间质的表达

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1、山东大学硕士学位论文质相对面积两两正相关：CTGF和HIF．1am0．980，CTGF和FN表达量r=0．963，CTGF和肾间质相对面积m0．966，HIF．1a和FNr=0．958，HIF．10【和肾间质相对面积r=0．971，FN表达量和肾间质相对面积r=0．961，以上均有P<0．01。结论：1．TGF—p1可能在纤维化的启动中起重要作用，纤维化的进行性发展，则还需要其他更重要因素的存在。2．CTGF介导TTGF．pl致纤维化的作用，参与肾间质纤维化发生发展的全过程，与CAAN大鼠肾间质纤维化有着更为直接的关系。3．HIF．1a通过与CTGF的相互

2、作用参与CAAN大鼠肾间质纤维化，是肾间质纤维化进行性发展的重要因素。关键词：马兜铃酸；肾间质纤维化；转化生长因子．p1；结缔组织生长因子；缺氧诱导因子．10‘；血管内皮生长因子；纤维连接蛋白3山东大学硕士学位论文EXPRESSIONOFCTGFANDHIF．1aiNTHERENALTUBULE．INTERSTITIUMOFRATSWITHCHRoNICObjectivesARISToLoCHICACIDNEPHRoLoGYPostgraduate：JIAXiaoyanSpeciality：NephrologyTutor：ProfessorXUDongme

3、iABSTRACT1．ToestablisharatmodelwithAristolochiamanshuriensisKomandobservethechangesofkidneytissuehistology．2．Todetectthekineticexpressionoftransforminggrowthfactor-131(TGF邛1)，connectivetissuegrowthfactor(CTGF)，hypoxiainduciblefactor-1a(HIF—la)，VaScularendothelialgrowthfactor(VEGF)

4、andfibronectin(F№intherenaltubule-interstitiumofrats、ⅣimChronicaristolochicacidnephrologyandtodiscusstheirinfluenceontherenalinterstitialfibrosisofCAANrats．Methods1．Thirty-sixmaleWistarratsweredividedrandomlyintotwogroups：modelgroup(n=24perfusedintragastricallywithdecoctionofCauli

5、saristolochiaemanchuriensisKomfor12weeks)andcontrolgroup(n212，perfusedintragastrically谢t11tapwaterforl2weeks)．Attheendsof4，8，12and16weeks，6ratsinmodelgroupand3ratsincontrolgroupwerekilled．Specimensofrenaltissueswerecollectedbeforethekillingoftheratsforfurthertest．2．HEandMassonstai

6、ningandmicroscopywereusedtoobservethehistologyofrenaltissues．Therelativeareaofrenalinterstitiumwasdetectedtoevaluatethedegreeofrenalinterstitialfibrosis．ImmunohistochemistrywereusedtodetecttheexpressionofTGF-I]I，CTGF,HIF—la，VEGFandFN．3．StatisticalsoftwareSPSS13．0Wasusedforstatisti

7、caltreatment．Measurementdataswererepresentedasx土，．T-testwasusedforinterclassdifferencetest．4山东大学硕士学位论文Pearsoncorrelationanalysiswasusedforcorrelationtest．Results1．Nopathologychangeswerefoundincontrolgroup．Incontrolgroup，tubulointerstitiallesionsbecamemoreandmoreseriousinmodelgroup

8、，attheendof16weeksallratsdevelope