可吸收诱导神经修复材料用于周围神经缺损的实验分析与初步临床应用 (1)

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1、ABSTRACTatrophyoftibialisanteriormusclesrecoveredslowlyaftertheoperationat3months．3．Electrophysiologicalassessment：At9monthsan口surgery,electrophysiologictestswereperformedOnallanimals．Singleelectricalpulsesweredeliveredviabipolarelectrodesplacedinturnattheproximalanddistaltrunkofthegrafte

2、dnerve．Thelatencyandamplitudewererecordedviaanelectrodeinsertedintotheanteriortibialmuscle．Thedifferenceconductionvelocitywascomputed．CMAPsweredetectedingroupsPRGD／PDLLA／p·TCP／NGFandautografl．ThedifferenceinCMAPamplitudewasnotstatisticallysignificantbetweengroupsPRGD／PDLLA／[3一TCP／NGFandau

3、tografl．(P>0．05)4．Histologicalobservation：ThetibialisanteriormusclesofgroupsPRGD／PDLLA／[3-TCP／NGFwereobservedbyHEstainingat3months，6months，9months．ThemusclefiberdiameterWassignificantlysmaller,hasmorenuclei，stainingreactionincreasing．Thereweremoreconnectivetissuebetweenmusclebundles．Theat

4、rophyoftibialisanteriormusclesrecoveredslowlyaftertheoperationat3months．At9monthscontinuousregeneratednervetissuelocatedintheconduit．Thesurfaceofregeneratednervecanbeseencreepinggrowthofnutrientvessels．Methylenebluestaining：Nervefibersevenlydistributedintheproximalofregeneratednerve．Themy

5、elinsheaththickerthanbefore．Therearemanyvaryingsizesofnervebundlesintheregeneratednerve．Betweenthenervebundlestherearerichinconnectivetissue．Themyelinsheathisthinnerthantheproximalofregeneratednerve．Thenervefiberswereregionalanddispersedinthedistalofregeneratednerve．Themorphologicalisregu

6、lar．Themyelinsheathisthinner．Quantitativem3ageanalysis：theconduitgroupandtheautologousgrouptwosetsofaxondiameterofmyelinatednervefibers,myelinthicknesscomparedwithnosignificantdifference(P>0．05)differenceswerenotstatisticallysignificant．UlWastructureundertransmissionelectronmicroscope：Mat

7、urenervefiberscanbeⅣ硕士学位论文seenintheproximalandcenterofthenerveconduitandthedistalnervestump．Theregeneratednerveischaracteristicsofclearmyelinlamellar,richmicrofilamentsmicrotubules．ItCanbefoundthattheSchwanncellssurroundingtheaxon．Therearepartofthemyelinatednervefib