可吸收诱导神经修复材料用于周围神经缺损的实验-.研究与初步临床应用

《可吸收诱导神经修复材料用于周围神经缺损的实验-.研究与初步临床应用》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。

1、ABSTRACTatrophyoftibialisanteriormusclesrecoveredslowlyaftertheoperationat3months．3．Electrophysiologicalassessment：At9monthsan口surgery,electrophysiologictestswereperformedOnallanimals．Singleelectricalpulsesweredeliveredviabipolarelectrodesplacedinturnatthepro

2、ximalanddistaltrunkofthegraftednerve．Thelatencyandamplitudewererecordedviaanelectrodeinsertedintotheanteriortibialmuscle．Thedifferenceconductionvelocitywascomputed．CMAPsweredetectedingroupsPRGD／PDLLA／p·TCP／NGFandautografl．ThedifferenceinCMAPamplitudewasnotsta

3、tisticallysignificantbetweengroupsPRGD／PDLLA／[3一TCP／NGFandautografl．(P>0．05)4．Histologicalobservation：ThetibialisanteriormusclesofgroupsPRGD／PDLLA／[3-TCP／NGFwereobservedbyHEstainingat3months，6months，9months．ThemusclefiberdiameterWassignificantlysmaller,hasmor

4、enuclei，stainingreactionincreasing．Thereweremoreconnectivetissuebetweenmusclebundles．Theatrophyoftibialisanteriormusclesrecoveredslowlyaftertheoperationat3months．At9monthscontinuousregeneratednervetissuelocatedintheconduit．Thesurfaceofregeneratednervecanbesee

5、ncreepinggrowthofnutrientvessels．Methylenebluestaining：Nervefibersevenlydistributedintheproximalofregeneratednerve．Themyelinsheaththickerthanbefore．Therearemanyvaryingsizesofnervebundlesintheregeneratednerve．Betweenthenervebundlestherearerichinconnectivetissu

6、e．Themyelinsheathisthinnerthantheproximalofregeneratednerve．Thenervefiberswereregionalanddispersedinthedistalofregeneratednerve．Themorphologicalisregular．Themyelinsheathisthinner．Quantitativem3ageanalysis：theconduitgroupandtheautologousgrouptwosetsofaxondiame

7、terofmyelinatednervefibers,myelinthicknesscomparedwithnosignificantdifference(P>0．05)differenceswerenotstatisticallysignificant．UlWastructureundertransmissionelectronmicroscope：MaturenervefiberscanbeⅣ硕士学位论文seenintheproximalandcenterofthenerveconduitandthedist

8、alnervestump．Theregeneratednerveischaracteristicsofclearmyelinlamellar,richmicrofilamentsmicrotubules．ItCanbefoundthattheSchwanncellssurroundingtheaxon．Therearepartofthemyelinatednervefib