资源描述:
《pten,akt,tgf-β1蛋白在瘢痕疙瘩中的表达》由会员上传分享,免费在线阅读,更多相关内容在工程资料-天天文库。
1、PTEN,Akt,TGF-β1蛋白在瘢痕疙瘩中的表达郭亮1,徐凯,章鑫,黄巧玲,桂思,晏洪波,李世荣2广州军区武汉总医院皮肤整形美容外科湖北武汉430070【摘要】目的::探讨瘢痕疙瘩组织中TGF-β1、第十号染色体同源丢失性磷酸酶基因(phosphataseandtensinhomologdeletedonChromosome10,PTEN)和蛋白激酶(Akt)的表达。方法::采用免疫组化SP法检测TGF-β1,,PTEN,和p-Akt在2028例瘢痕疙瘩,及17例增生性瘢痕及1240例正常皮肤组织中的表达水平。结果::瘢痕疙瘩组织中,TGF-β1,p-A
2、kt蛋白阳性表达率分别为43.94±7.59以及44.95±6.6567.50%,70.00%,均高于增生性瘢痕及正常皮肤组(Pp<0.0167);PTEN蛋白阳性表达率为6.53±1.4525.00%,低于其他两正常皮肤组(Pp<0.0167)。PTEN的表达与p-AktTGF-β1,TGF-β1Akt的表达强度成负相关(r=-0.6437,-0.376p<0.05,r<0),TGF-β1,与p-Akt表达成正相关(r=0.804p<0.05,r=0.63)。结论::PTEN,,Akt及TGF-β1在瘢痕疙瘩的发生发展中均起着重要的调控作用,ILK,PI3
3、K及PTEN可能依赖PTEN/PI3K/ILK/PKBAkt信号传导通路可能通过调控TGF-β1共同参与病理性瘢痕瘢痕疙瘩的形成,且ILK,PI3K可能起协同作用,与PTEN互相拮抗。关键词:瘢痕疙瘩,PTEN,p-Akt,TGF-β1ExpressionsofPTEN,AktandTGF-β1proteininkeloidSONGNing-ning,NIUFu-you,WANGXi-mei,ZHAIXiao-mei,MAXiu-yun,YUANDe-pinGuoLiang,XuKai,ZangXin,HuangQiaoling,GuiSi,Yanhongb
4、o,LiShirong(DepartmentOfPlasticSurgery,WuhanGeneralHospitalofGuangzhouMilitaryRegiontheFirstAffiliatedHospitalOfZhengzhouUniversity,ZhengzhouWuhan,450052430070,HenanHubei,China)Abstract:Objective:ToinvestigatetheexpressionsofTGF-β1ILK,p-PI3KAktandPTENproteinsinpathologicscarkeloid.
5、Methods:SPimmunohistochemicalmethodwasusedtodetecttheexpressionsofTGF-β1ILK,p-PI3KAktandPTENproteinsin1郭亮,1979年2月,整形外科博士,研究方向:创面愈合与病理性瘢痕,lidaoziyuan@yahoo.com.cn2通讯作者,第三军医大学西南整形美容专科医院,博士生导师4028casesofpathologicscar,20casesofnon-pathologicscarand20casesofnormalskintissue.Results:The
6、positiveratesofTGF-β1ILKandp-AktPI3Kproteinsin4028casesofkeloidpathologicscarwere43.94±7.59and44.95±6.65,67.50%,70.00%andwerehigherrespectivelycomparedwiththetwocontrolgroups(PP<0.0167);TherateofPTENproteinsinpathologicscarskeloidgroupwas6.53±1.4525.00%,significantlylowerthantheoth
7、ersnormalskingroup(PP<0.0167).AnegativecorrelationwasfoundbetweentheexpressionofPTENandp-AktPI3KorTGF-β1ILKproteins(r=-0.6437,-0.376P<0.05)inpathologicscarkeloid,andtherewasahighlypositivecorrelationbetweenTGF-β1PI3Kandp-AktILKproteinexpression(r=0.804P=0.000,r=0.63).Conclusion:PTE
8、N,AktILK,PI3KandTGF-β1PTEN