sbh2对谷氨酸单钠所致小鼠神经毒性的保护作用

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1、鼠及其仔鼠Y迷宫、水迷宫学习和记忆能力的损伤作用；逆转中枢神经系统过度兴奋；改善对探究好奇行为的抑制和对高空协调运动能力的损伤．4．SBH-2能够显著降低DL-[2，3)H]Glu在脑组织中的含量。5．SBH-2a系从SBH-2中分离纯化的单体成分，SBH-2a能显著减少MsG在脑组织中的分布，提示SBH-2a是神经保护剂SBH-2的有效成分．6．本实验结果表明，过量谷氨酸单钠(MsG)对小鼠及其仔鼠具有神经毒性作用；SBH-2及其有效成分SBH-2a对谷氨酸单钠的神经毒性具有保护作用．关键词谷氨酸单钠，神经毒性，保护作用，昆明小鼠111Protectiveeff

2、ectsofSBH一2onMSG—inducedneurotoxicityingliceABSTRACTBackgroundand0bjective：Excessiveamountsofglutamatehavedeleteriouseffectsonneurons,whichistermed勰excitotoxicity．Glutamateisaprincipalexcitatoryneurotransmitterincentralnervoussystem(CNS)ofhumanandmammalanimals．．11壕possibilitythatsomea

3、cidicaminoacidsoccurringnaturally,or鹊additivesinthediet．canactasexcitotoxinsproducillgCNSpathologyhasbeenthesubjectofextensivedebatefrom1960s．Theobservationscollectivelycons廿uedthatexcessiveamountsofintrinsicexcitatoryaminoacid(E从)，gJutamate(Glu)，wcrecapableofexcitingCNSncuronstosucha

4、nextentastoultimatelykillOh-sensitivena叫螂throughover-excitation．Oh-inducedneurotoxicityhasbeensuggestedtObeinvolvedinpathologicalmechanismsofsomediseasessuchasischemieorhypoxicdiseases,Parkinson'$disease(PD)。Huntington'sdisease(HI))，AIzhcimcr'sdisease(M))orAmyotmphicLmeralSclerosis(AL

5、S)，na1．ExogenousGlualsohasexcitotoxiceffectsonCNS，whichhasbeenreponedrepeatedly．11"presentstudywasdesignedtostudythepromcliveeffectsofSBH-2onmonosodiumzlutm斌e(MSG)-inducedncurotoxicityinmice．Methods：1．Onehundred8-wevk-oldKunmingmiceweredividedinto10groups鹧follows：2groupswercgivenMSG(2

6、．09／kgand4．09／kgbodyweight,ig)。3groupsWerCgivenMSG(2．09／kg．ig)andSBH-2(Sg／kg、lOg／kg、209／kgbodyweight,ig)atthesametime，3groupswercgivenMSG(4．Og／kg，ig)andSBH-2(109／kg、209／kg、309／kgbodyweight,ig)atthesanletime，onegroupwcregivenSBH-2(309／kgbodyweight．ig)aloneandthecontrolweregivennormalsa

7、lineeveryday．AndtheirbehaviorsWCqrcstudiedthereafter．Pathologicalobservationofthemicebrainwereusedinthisstudy,theotherbehaviorsofmicewercstudiedbytestofY-maze,waterm线measuresoflocomotoractivity,hole-boardandrope-climbing．Measurementofbody-weightgainandtestof托pIDI如cti、碍capacitywe”alsoc

8、arrie