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1、不同脂肪酸饮食对Wistar大鼠胰岛素敏感性的影响作者:李萍赵晓晖宋光耀王子婧马博清李文丛【摘要】目的探讨饱和脂肪酸(SFA)、单不饱和脂肪酸(MUFA)、多不饱和脂肪酸(PUFA)饮食对大鼠胰岛素敏感性的影响。方法48只雄性Wistar大鼠随机分为正常对照组、SFA组、MUFA组、PUFA组。正常对照组给基础饲料(脂肪占10.3%);SFA组饲料在基础饲料中添加15%猪油;MUFA组饲料在基础饲料中添加15%茶油;PUFA组饲料在基础饲料中添加15 %豆油(脂肪占35.4%)。8w后4组各随机选8只大鼠行高胰岛素-正葡萄糖钳夹实验,同时留取空腹血清测定血脂、胰
2、岛素等指标。结果实验第8周末,与其他3组相比,正常对照组大鼠进食量有所增加,差异有统计学意义(P>0.05)。除外进食量的影响,与SFA组相比,正常对照组、MUFA组、PUFA组血清TC、TG降低,差异有统计学意义(P<0.05),而正常对照组、MUFA、PUFA三组间无统计学意义(P>0.05)。与正常对照组、MUFA组相比,SFA、PUFA组FINS、FPG升高,差异有统计学意义(P<0.05),GIR明显下降,差异有统计学意义(P<0.05),但该两组间无统计学差异(P>0.05)。与正常对照组相比,MUFA组血清FINS
3、、FPG有升高趋势,差异无统计学意义(P>0.05),GIR下降,差异有统计学意义(P<0.05)。结论MUFA饮食较PUFA、SFA饮食可以改善胰岛素敏感性。10【关键词】饮食;胰岛素敏感性;单不饱和脂肪酸;多不饱和脂肪酸;饱和脂肪酸 【Abstract】ObjectiveToexploretheeffectsofsaturatedfatsacid(SFA)diets,polyunsaturatedfatsdietsacid(PUFA)andmonounsaturatedfatsacid(MUFA)dietsoninsulinsensitivityi
4、nrats.Methods48Wistarratswererandomlydividedintonormalcontrol(N),SFA,MUFA,PUFAgroups,eachgrouphad12rats.Theratsincontrolgroupwerefedwithregularlowfattyacidsdietcontaining10.3%fat,24.2%protein,and65.5%carbohydrateaspercentageoftotalcalories.TheratsinMUFA,PUFAandSFAgroupswerefedregulard
5、ietsmixedwith15%teaoil,15%soybeanoil,15%lardoilrespectively,containing35.4%fat,17.4%prot einand47.2%carbohydrateasapercentageoftotalcalories.Thebloodsamplewascollectedbycardiacpunctureafterratswereanesthetizedwithdiethyletherforthebiochemicalanalysis,insulinresistancewasevaluatedbygl
6、ucoseinfusionrate(GIR)ofhyperinsulinemiceuglycemicclamptechnique.ResultsComparedwiththatofNgroup,theamountsoffoodintakeweresignificantlylowerinSFA,MUFAandPUFAgroups(P<0.05).Adjustedfortheamountoffoodintake,10comparedwiththoseofSFAgroup,thelevelsofserumTC,TGweresignificantlylowerinN
7、,MUFAandPUFAgroups(P<0.05),buttherewasnodetectabledifferenceamongN,PUFAandMUFAgroups(P>0.05).ComparedwiththoseofNandMUFAgroups,thelevelsofFINSandFPGweresignificantlyhigherinSFAandPUFAgroups(P<0.05),GIRwassignificantlylowerinSFAandPUFAgroups(P<0.05).ComparedwiththatofNgroup
8、,GIRw