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1、TheeffectsandmechanismofthedifferentlevelsofinsulinontheaortainratsunderhighfatdietAbstractObjective:Toinspectthechangeofintima-mediathicknessofaortaunderhighfatdietratswithhyperinsulinemia,andtoinvestigateit’smechanismsbysettingdifferentinsulinlevelsinSpragueD
2、awleyratsfedwithhighfatdiet.Methods:Atotal32maleSDrats,health,aged8weeks,weight180-200g,wererandomlydividedintofourgroups:theNDgroupwasfedwithstandarddiet;theHFDgroupwasfedwithhighfatdiet;theHFD+INSgroupwasfedwithhighfatdietandtreatedbyinsulin(INS);theHFD+STZgr
3、oupwasfedwithhighfatdietandtreatedbystreptozotocin(STZ).After1weekofacclimationperiodandabrosiafor12hours,theHFD+STZgroupwasinducedwithasingleintraperitonealinjectionofSTZ(50mg/kg),othergroupswereinjectedbufferatthesamedoseinstead.Threedayslater,theHFD+INSgroup
4、wasinducedwithsubcutaneousinjectionof1U/Kglong-actinginsulineverymorning,othergroupswereinjectednormalsaline(NS)atthesamedoseinstead.Thechangesofbloodsugarandweightofratsweremonitored.UsedofcolordopplerultrasoundevaluationIMToftheaorta.Themorphologicalchangesof
5、aortatissueswereobservedthroughlightmicroscopyafterHEstaining.thelevelsofbodyweight,FPG,TG,LDL-C,TC,HDL-C,FINS,HbA1c%weremeasuredafter4months.TheHOMA-IRwasevaluated.TheexpressionofVEGF,TNF-ɑ,PGC-1ɑ,ApoEwereanalyzedbywesternblot.Results:1.ComparedwiththeNDgroup,
6、theIMTingroupsofHFDandHFD+INSwereincreasedforabout1.53foldand1.92fold(P<0.05).TheresultwasalsofoundstatisticallysignificantwhenHFDgroupwascomparedwithHFD+INSgroup.TheIMTwasnoobviousdifferenceinthegroupHFD+STZandND(P>0.05),buttheIMToftheHFD+STZgroupwasthinnerwhe
7、ncomparedwithHFDgroupandHFD+INSgroup(P<0.05).TheresultsoflightmicroscopyafterHEstainingwerethe5万方数据sameasabove,theIMTinHFD+INSgroupwasthethickest.Atthemagnification,thevascularendothelialwasincompleteandsomefoamyhistiocytescouldbeensaw.Butnotypicalatherosclerot
8、icplaqueswereobservedinallgroups.2.Thelevelsofbodyweight,TG,TC,LDL-C,FINS,HOMA-IRwereincreasedintheHFDandHFD+INSgroupscomparedwiththeNDgroup(P<0.05).WhencomparedwiththeHFDgr
