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1、中国汉族男性CYP17基因多态性与前列腺癌发生危险性的关系512?泌尿外科杂志2oo48旦箜鲞箜塑!:旦:!::!:中国汉族男性CYP17基因多态性与前列腺癌发生危险性的关系王军起艾军魁古力米热李鸿伟刘建河佟明袁亦铭辛殿祺李鸣那彦群【摘要】目的探讨CYP17基因多态性与前列腺癌发生危险性的关系.方法提取125例前列腺癌患者和158例对照组的血DNA标本,设计引物通过PCR技术扩增出包括基因多态位点的片段,用限制性内切酶MspA1I进行酶切,产物在2%琼脂糖凝胶上电泳,确定出CYP17基因的3种基因型,即A1/A1,A1/A2,A2/A2,并经测序证实.结果基因型A1/A
2、2,A2/A2频数与A1/A1基因型进行比较,A1/A2和A2/A2在前列腺癌中的频数与对照组相比,其OR值分别为1.19和1.28,P值分别为0.57和0.45;但年龄>69岁的病例中,其OR值分别为3.97和3.21,P值分别为0.007和0.023;对照组的血fPSA,tPSA水平3组间差异无显着性意义.结论A1/A2,A2/A2基因型未增加汉族人群前列腺癌发生的危险性,基因型间血PSA水平差异无显着性支持这一观点.年龄>69岁的人群中,A1/A2,A2/A2基因型显着增加前列腺癌发生的危险性,提示CYP17基因可能与老年男性前列腺癌发生危险性之间有密
3、切关系.【关键词】前列腺肿瘤;癌;基因;多态性AssociationbetweenthepolymorphismofCYP17geneandriskofprostatecancerinChinesemenWANGJun-qi,GULlMi-re,LIHong-wei,eta1.InstituteofUrology,PekingUniversity,Beqing100034,China【Abstract】0bjectiveToinvestigatetheassociationbetweenthepolymorphismofCYP17geneandtheriskofpros
4、tatecancer.MethodsAcase-controlstudyincluding125prostatecancerpatientsand158age?matchedcontrolswasconducted.ThepolymorphismwasinvestigatedbyPCRusingDNAfromperipheralbloodlymphocytes.Thetransition(T-+C)intheriskallele(A2)producedanewrecognitionsitefortherestrictionenzymeMspA1.Threegenotyp
5、esofCYP17gene(A1/A1,A1/A2,A2/A2)weredeterminedandconfirmedbysequencing.ResultsThefrequencyoftheA2allelewassimilarinthecasesandcontrols.ComparedwithmenwithA1/A1genotype.theoddsratioswere1.19and1.28fortheA1/A2andA2/A2genotypes,respectively.However,stratificationbyageofprostatecancercasesre
6、vealedthatamongprostatecancercasesolderthan69years,theoddsratiowas3.97and3.21fortheA1/A2andA2/A2genotypes(P=0.007,0.023,respectively).Meanwhile,comparisonamong3subgroups(divisionbygenotypes)ofcon-trols,thefPSAandtPSAlevelswerenotsignificantlydifferent.ConclusionsTheseresultsindicatethatA
7、2alleledoesnotincreasetheriskofprostatecancerinChinese.Butinmenolderthan69years,A1/A2andA2/A2genotypespredictsusceptibilitytoprostatecancer.ItissuggestedthatCYP17genemaybeasSO-ciatedwiththemorbidityofprostatecancerinelderlymalepopulation.【Keywords】Prostaticneoplasms;Carci
