川芎嗪衍生物tbn的hplc含量测定方法

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1、QuantitativeHPLCAnalyticalMethodforTetramethylpyrazineDerivativeTBNStudentNameMAKSAUHANGStudentNo.MajorSupervisorProfessorWang,YuqiangDate(dd/mm/yyyy)暨南大学本科生毕业论文论文题目川芎嗪衍生物TBN的HPLC含量测定方法学院国际学院学系药学系专业药学姓名学号指导教师StatementofOriginalityIherebydeclarethatthethesispresentedist

2、heresultofresearchperformedbymepersonally,underguidancefrommysupervisor.Thisthesisdoesnotcontainanycontent(otherthanthosecitedwithreferences)thathasbeenpreviouslypublishedorwrittenbyothers,nordoesitcontainanymaterialpreviouslypresentedtoothereducationalinstitutionsford

3、egreeorcertificatepurposetothebestofmyknowledge.Ipromisethatallfactspresentedinthisthesisaretrueandcreditable.Signed:_________________Date：QuantitativeHPLCanalyticalmethodfortetramethylpyrazinederivativeTBNAbstract:Ahigh-performanceliquidchromatography(HPLC)methodforan

4、ewtetramethylpyrazinederivative,TBN,wasdeveloped.Analyticalperformanceparameterssuchaslinearity,precision,accuracy,specificity,limitofdetection(LOD)andlimitofquantification(LOQ)weredeterminedundertheguidelineofInternationalConferenceonHarmonizationQ2B[1].TBNwasanalyzed

5、byRP-HPLCwithC18columnusingmethanol-water(35:65)asmobilephase.Theflowrateis0.8ml/minandthedetectorwassetto295nm.Thelinearityofcalibrationcurveisgood(r2>0.999)andtheLODandLOQwere12.096ng/mland40.32ng/mlrespectively.Therelativestandarddeviation(RSD)ofprecisionandaccuracy

6、were0.1245%and0.6895%,respectively,andthesamplerecoverywas99.08%(RSD:0.40%).ThismethodisreliableandeasyforTBNanalysis.KeyWords:tetramethylpyrazinederivative,TBN,HPLC川芎嗪衍生物TBN的HPLC含量测定方法摘要：目的：建立川芎嗪衍生物(TBN)高效液相含量测定方法。方法：选用C-18色谱柱；甲醇-水(35:65)；流速：0.8ml/min；检测波长为295nm。结果：TB

7、N在12.42-310.5mg/ml范围内线性关系良好(r2>0.999)；定量限和检测限分别为12.096ng/ml及40.32ng/ml；精密度及准确度RSD分别为0.1245%及0.6895%；加样回收率结果为99.08%(RSD:0.40%)。结论：本方法简便可靠，可作为TBN之定量分析方法。关键词：川芎嗪衍生物，TBN，高效液相Contents1.Introduction32.Materials32.1.Chemicals32.2.Apparatus33.ExperimentalMethod33.1.TBNstandard

8、preparation33.1.1.ColumnchromatographyforTBNpurification33.1.2.Semi-preparativecolumnchromatography33.2.Developmentof