芍药花提取物对果蔬腐败菌的抑菌活性及其在樱桃番茄保鲜中的应用

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1、芍药花提取物对果蔬腐败菌的抑菌活性及其在樱桃番茄保鲜中的应用李雯霞于司达诸葛斌方慧英宗红陆信曜孙进冯倩江南大学糖化学与生物技术教育部重点实验室江南大学工业生物技术教育部重点实验室生物工程学院工业微生物研宄中心浙江省调味食品制造工程技术研宄中心以苟药(Paeonialactiflora,P.lactiflora)花取物为研宄对象，通过抑菌圈、最低抑制浓度及最低致死浓度评价对主要果蔬腐败菌的抑菌性能;通过提取物对枯草芽孢杆菌(Bacillussubtilis,B.subtilis)生长、电导率、内容物质、可溶性糖的影响探宂芍药花提取物的抑菌方式;对腐败率、失重率、

2、可溶性岡形物及Vc含量的测定考察提取物对樱桃番茄的保鲜效果。结果表明：芍药花提取物对细菌、酵母均有很强的抑制作用，尤其对枯草芽孢杆菌最低抑制浓度和最低致死浓度分别低至2mg/mL和4mg/mL;提取物通过增加细胞膜的通透性及破坏细胞膜的结构发挥抑菌作用；与对照相比，芍药花提取物能够明显降低樱桃番茄腐败率和失重率，减少可溶性固形物及Vc的损失，是一种具有应用前景的优良保鲜剂。关键词：芍药花提取物;最低抑制浓度;抑菌活性;细胞膜;保鲜;基金：国家科技支撑计划(2015BAD16B00,2015BAD16B01)AntimicrobialactivityofPaeo

3、nialactifloraflowerextractsonfruitandvegetablespoilageorganismsanditsapplicationincherrytomatopreservationLIWen-xiaYUSi-daZHUGEBinFANGHui-yingZONGHongLUXin-yaoSUJinFENQianTheKeyLaboratoryofCarbohydrateChemistryandBiotechnology，MinistryofEducation，JiangnanUniversity;EngineeringResear

4、chCenterofCondimentsIndustryofZhejiangProvince;Abstract：TheantibacterialactivityofPaeonialactifloraflowerextractsonfruitandvegetablespoilageorganismswasinvestigatedbydeterminingthediameterofinhibitionzone,minimuminhibitoryconcentrationandminimumbactericidalconcentration.Antibacteria

5、lmodewasstudiedbasedonthecellgrowth,conductivity,contentandsolublesugarofBacillussubtilis.Fresh-keepingeffectoncherrytomatoesofP.lactifloraflowerextractswasstudiedbydeterminingtherateofcorruption,weightloss,solublesolidsandVccontent.TheresultsshowedthattheP.lactifloraflowerextractsh

6、adastronginhibitoryeffectonbacteriaandyeasts.TheminimuminhibitoryandminimumbactericidalconcentrationstoB.subtiliswereespeciallyaslowas2mg/mkand4mg/mL,respectively.Theextractsexertsabacteriostaticeffectbyincreasingthepermeabilityanddestroyingthestructureofthecellmembrane.Comparedwith

7、thecontrol,theP.lactifloraflowerextractscanobviouslyreducethecorruptionrateandweightlossrateofcherrytomatoes,andreducethelossofsolublesolidsandVc,whichisanexcellentpreservativewithpromisingapplication.Keyword：Paeonialactifloraflowerextracts;minimuminhibitoryconcentration;antibacteri

8、alactivity;cellmemb