空肠弯曲杆菌感染对鸡胚肠上皮细胞tlr1和tlr15mrna转录水平的影响

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1、2014୍36ज22௹ChinaPoultryVol.36đNo.22.2014ൌဒ࿹࣮◎◎ყٝ൵၄◎◎ॢӋຕ౷ۆऩۋಙؓ࠙஠Ӌഈ௃༥ІTLR1ބTLR15mRNAሇ੣ඣ௜႕ཙ*1đ21đ21đ21đ21đ21đ21đ2**น૓बđᅦཬဉđนྩबđᅦ࣡đ௰म޿đ۫౩৳đۚზൈč1.୪ြࡅౚ௖ᇉࡓ׼࡟ဒҩ൫ᇏྏđࡾ්ဘᇜ225125Ġ2.ࡾ්സࡅౚ॓࿐࿹࣮෮đࡾ්ဘᇜ225125Ďᅋေğ࿹࣮ၛ࠙஠Ӌഈ௃༥Іູଆ྘đҐႨཌྷۆ౷ຕӋॢҩ࡟ඌ࠯RCPਈקܻ႐ؓऩۋಙؓ࠙஠Ӌഈ௃༥ІTollဢ൳ุčTLRĎ1ބ15mRNAሇ੣ඣ௜႕ཙđฐ

2、ษTLR1ބTLR15ᄝॆॢӋຕ౷ۆऩۋಙᇏቔႨࠣఃࠏᇅbࢲݔཁൕğᆞӈ࠙஠Ӌഈ௃༥Іି࡟ҩTLR1ބTLR15mRNAሇ੣ĠॢӋຕ౷ۆऩۋಙ༥ІުđTLR1ᄝ3hުሇ੣ඣ௜ิۚđ12hղۚڂĠTLR15ᄝۋಙު6hሇ੣ඣ௜ղۚڂđᇀ12h੻Ⴕ༯ࢆĠᄝᆜ۱൫ဒᇛ௹ۋಙቆTLR1ބTLR15mRNAሇ੣ඣ௜׻ࠞཁᇷۚႿؓᅶቆčP<0.01Ďbၛഈ࿹࣮іૼTLR1ބTLR15აӋഈ௃༥ІॆॢӋຕ౷ۆऩۋಙ૧ၦّႋऎႵཌྷܱྟđTLRᄝ༥Іᇏіղ൐ھ༥ІႵି৯პຓࢸ؟ᇕѨჰັള໾ೆ౓bܱ࡯ՍğॢӋຕ౷ۆऩĠ࠙஠Ӌഈ௃༥ІĠTollဢ൳ุ

3、EffectsofCampylobacterjejuniInfectiononToll-likeReceptor1and*15mRNAExpressioninChickenEmbryoIntestinalEpithelialCells1đ21đ21đ21đ2TANGMengjunđZHANGXiaoyanđTANGXiujunđZHANGJingđ1đ21đ21đ2**PUJunhuađGEQinglianđGAOYushič1.QualityInspectionCenterofPoultryđMinistryofAgricul

4、tuređYangzhouđJiangsu225125Ġ2.JiangsuInstituteofPoultryScienceđYangzhouđJiangsu225125ĎAbstractğChickenembryointestinalepithelialcellswereusedasthemodeltoinvestigatetheeffectofCampylobacterjejuniinfectiononToll-likereceptorčRLRĎ1and15mRNAexpressionandfurthertoexplorei

5、ntestinalinnateimmuneresponsestothebacteriainthisstudy.CulturedchickenembryointestinalepithelialcellswereinfectedbyCampylobacterjejunifor0to12hđthemRNAlevelsofTLR1andTLR15weredetectedusingreal-timePCR.Theresultsshowedthatcontrolledchickenembryointestinalepithelialcel

6、lsexpressedTLR1andTLR15mRNAĠTLR1andTLR15geneincreasedasearlyas3hinresponsetoCampylobacterjejuničP<0.01ĎĠTheheightofTLR1andTLR15geneexpressiongotthepeakafter12hand6hđrespectively.SignificantincreaseinTLR1andTLR15geneexpression൬۠ರ௹ğ2014-08-06ྩ߭ರ௹ğ2014-09-15*ࠎࣁཛଢğࡾ්സሱಖ॓

7、࿐ࠎࣁཛଢčBK2010297Ď**๙࿟ቔᆀđE-mailğgaoys100@sina.com-16-2014୍36ज22௹ChinaPoultryVol.36đNo.22.2014ൌဒ࿹࣮weredetectedinresponsetoCampylobacterjejuni.ItwasevidentthatTLR1andTLR15geneexpressionassociatedwithCampylobacterjejuniinfection.TheresultsindicatedthatTLRgeneexpressioninc

8、hickenembryosintestinalepithelialcellshavetheabilitytoresisttheinvasionofavarietyofpathogenicmicroorganisms.KeywordsğCampylobacterj