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1、中国水稻科学(ChinJRiceSci),２０１６,３０(４):４０６－４１６http://www．ricesci．cn４０６DOI:１０．１６８１９/j．１００１Ｇ７２１６．２０１６．６０２６类酵母共生菌中两个组氨酸合成基因在褐飞虱发育中的作用唐耀华１,２万品俊２郝培应１傅强２,∗俞晓平１,∗(１中国计量大学生命科学学院浙江省生物计量与检验检疫技术重点实验室,杭州３１００１８;２中国水稻研究所水稻生物学国家重点实验室,杭州３１０００６;∗通讯联系人,EＧmail:fuqiang＠caas．cn;yxp＠cjlu．edu．cn)RolesofTwoGenesInvolvedinHistidin
2、eBiosyntheticPathwayinYeastＧLikeSymbiontinDevelopmentofNilaparvatalugens(Stål)TANGYaoＧhua１,２,WANPinＧjun２,HAOPeiＧying１,FUQiang２,∗,YUXiaoＧping１,∗(１ZhejiangProvincalKeyLaboratoryofBiometrologyandInspectionandQuarantine,CollegeofLifeScience,ChinaJiliangUniＧversity,Hangzhou３１００１８,China;２StateKeyLaborator
3、yofRiceBiology,ChinaRiceResearchInstitute,Hangzhou３１０００６,ChiＧna;∗Correspondingauthor,EＧmail:fuqiang＠caasc．n;yxp＠cjlue．duc．n)TANGYaohua,WANPinjun,HAOPeiying,etal．RolesoftwogenesinvolvedinhistidinebiosyntheticpathwayinyeastＧlikesymbiontindevelopmentofNilaparvatalugens(Stål)．ChinJRiceSci,２０１６,３０(４):４０６
4、Ｇ４１６．Abstract:NilaparvatalugensisaseriousphloemＧfeedingpestofriceinChina．BasedongenomeandtranscriptomedataofN．lugensandyeastＧlikesymbiont(YLS,alsonamedEntomomycesdelphacidicolastr．NLU),themajorbiosynthesispathwaysforaminoacidsinNl．ugenswereconstructed．Inthisstudy,weclonedtwogenes,EdeHis２andEdeHis６,w
5、hichcatalyzecriticalstepsinhistidinebiosynthesispathway,andrevealedthenegativeeffectsofdoubleＧstrandedRNA(dsRNA)onthegrowth,developmentandsurvivalrateofN．lugens．HomologysearchesandphylogeneticanalysisshowedthatEdeHis２andEdeHis６originfromYLSgenome,sharehighsimilaritieswiththatofMetarhiziumacridumandf
6、ormedacladinthephylogenetictree,whereasnoHis２ＧorHis６ＧlikegeneswasfoundinNl．ugensgenome．TemporalexpressionprofilesofEdeHis２andEdeHis６showedthatbothgeneswereubiquitouslybutunevenlyexpressedamongthedifferentlifestages,andthespatialexpressionpatternshowedtheyhavehigherexpressionlevelsinthefatbodyrathert
7、hanhead,leg,integumentandmidgut．Furthermore,notargetproductswereamplifiedinheadandwinggenomicDNAofN．lugens,ratherthanthatinabdomengenomicDNA．Attwo,fourandsixdaysafterdsEdeHis２ordsEdeHis６injection,them
