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1、中国水稻科学(ChinJRiceSci),２０１５,２９(１):９７－１０５http://www．ricesci．cnDOI:１０．３９６９/ji．ssn．１００１Ｇ７２１６．２０１５．０１．０１２９７水稻苗期纹枯病抗性鉴定微室接种技术的改良徐国娟１,２袁正杰２左示敏３潘学彪３王志龙４瞿绍洪２,∗(１浙江师范大学化学与生命科学学院,浙江金华３２１００４;２浙江省农业科学院,病毒学与生物技术研究所,杭州３１００２１;３扬州大学江苏省作物遗传生理重点实验室/教育部植物功能基因组学重点实验室,江苏扬州２２５００９;４湖南农业大学湖南省作物种质创新与资源利用重点实
2、验室,长沙４１０１２８;∗通讯联系人,EＧmail:squ＠mail．zaas．ac．cn)ImprovementoftheMicroＧchamberInoculationMethodforDeterminationofRiceSeedlingResistancetoSheathBlight(Rhizoctoniasolani)XUGuoＧjuan１,２,YUANZhengＧjie２,ZUOShiＧmin３,PANXueＧbiao３,WANGZhiＧlong４,QUShaoＧhong２,∗１(CollegeofChemistryandLifeScience
3、s,ZhejiangNormalUniversity,Jinhua３２１００４,China;２InstituteofVirologyandBiotechnology,ZhejiangAcademyofAgriculturalSciences,Hangzhou３１００２１,China;３KeyLaboratoryofCropGeneticsandPhysiologyofJiangsuProvince,CollegeofAgriculture,YangzhouUniversity/KeyLaboratoryofPlantFunctionalGenomicsof
4、theMinistryofEducation,Yangzhou２２５００９,China;４HunanProvincialKeyLaboratoryofCropGermplasmInnovationandUtilizationandCollegeofAgronomy,HunanAgriculturalUniversity,Changsha４１０１２８,China;∗Correspondingauthor,EＧmail:squ＠mail．zaas．acc．n)XUGuojuan,YUANZhengjie,ZUOShimin,etal．Improvementof
5、themicroＧchamberinoculationmethodfordeterminationofriceseedlingresistancetosheathblight(Rhizoctoniasolani)．ChinJRiceSci,２０１５,２９(１):９７Ｇ１０５．Abstract:Thesheathblightresistanceinricebelongstoatypicalquantitativetrait,andmostofthericecultivarsshowlowlevelsofresistancetothediseasewithsm
6、alldifferences．Theinoculationandscoringsystemforricesheathblightisanimportantbasisforbreedingresistantricecultivars．ThemicroＧchambermethodfordeterminationofriceresponsestothesheathblightpathogenRhizoctoniasolaniwasimprovedbyinoculationtoriceseedlingswithsimilargrowthvigourundercon
7、trolledillumination,temperatureandhumidityconditionsinaplantgrowthchamber．ThericecultivarsYSBR１,Teqing,Taijing３９４,NipponbareandLemontshowedhightolowlevelsofsheathblightresistance,whichwasconsistentwiththeresultsoffieldinoculationinadultriceplants．RTＧPCRanalysisshowedthattheexpress
8、ionoffourricegenesrelatedtodiseas
