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1、中国水稻科学(ChinJRiceSci),２０１４,２８(２):１１１－１１８http://www．ricesci．cnDOI:１０．３９６９/ji．ssn．１００１Ｇ７２１６．２０１４．０２．００１１１１水稻苗期低温白叶突变体cde２的鉴定和基因定位魏祥进１,＃宋建１,２,＃刘胜１邵高能１圣忠华１唐绍清１胡培松１,∗(１中国水稻研究所水稻生物学国家重点实验室,杭州３１０００６;２浙江省农业科学院,杭州３１００２１;＃共同第一作者;∗通讯联系人,EＧmail:hupeisong＠caas．cn)IdentificationandGeneti
2、cMappingofaThermoＧsensitiveWhiteLeafMutantinRiceWEIXiangＧjin１,＃,SONGJian１,２,＃,LIUSheng１,SHAOGaoＧneng１,SHENGZhongＧhua１,TANGShaoＧqing１,HUPeiＧsong１,∗(１StateKeyLaboratoryofRiceBiology,ChinaNationalRiceResearchInstitute,Hangzhou３１０００６,China;２ZhejiangAcademyofAgriculturalScience
3、s,Hangzhou３１００２１,China;＃Theseauthorscontributedequallytothispaper;∗Correspondingauthor,EＧmail:hupeisong＠caasc．n)WEIXiangjin,SONGJian,LIUSheng,etal．IdentificationandgeneticmappingofathermoＧsensitivewhiteleafmutantinrice．ChinJRiceSci,２０１４,２８(２):１１１Ｇ１１８．Abstract:AthermoＧsensi
4、tivechlorophylldeficientmutantwasobtainedfromtheprogenyoftissuecultureofajaponicaricecultivarAsominori．Themutantwascharacterizedbyalbinoleavesbefore３Ｇleafstageunderlowtemperature(２３℃),whileshowingnormalphenotypeunderhightemperature(３０℃)．Thechlorophyllcontentsofthemutantwer
5、esignificantlylowerthanthoseofwildＧtypebefore３Ｇleafstageunderlowtemperature,whichisduetoalteredchloroplastdevelopmentunderlowtemperature．Geneticanalysisshowedthatthemutationwascontrolledbyasinglerecessivegene,whichistentativelydesignedascde２(chlorophylldeficient２)．Furtherm
6、ore,usinganF２populationderivedfromcde２/Peiai６４andpolymorphicmarkersbetweencde２andPeiai６４,thecde２wasfinallylocalizedonthecentromericregionofchromosome１andpositionedbetweenSSRmarkersRM１１０４１andIndel１,withinthephysicaldistanceabout３６５．６kb．Inaddition,theexpressionlevelofgenesas
7、sociatedwithchlorophyllbiosynthesis,photosynthesisandplastidialtranscription/translationapparatuswasmeasuredandtheresultsshowsthatthecde２mutationincreasedthetranscriptlevelofchlorophyllbiosynthesisgenesandtheplatidialtranscription/translationapparatusrelatedgenes,butreduce
8、dtheexpressionlevelofphotosynthesisgenes,indicatingthatCDE２mayinvolveinthechlorophyllbios
