资源描述：
《水稻矮秆多分蘖突变体bf370的遗传分析和基因定位》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、中国水稻科学(ChinJRiceSci),２０１５,２９(４):３５７－３６２http://www．ricesci．cnDOI:１０．３９６９/ji．ssn．１００１Ｇ７２１６．２０１５．０４．００４３５７水稻矮秆多分蘖突变体bf３７０的遗传分析和基因定位胡运高１,２杨国涛２郭连安２钦鹏１陈永军２李仕贵１,∗(１四川农业大学水稻研究所,成都６１１１３０;２西南科技大学水稻研究所,四川绵阳６２１０１０;∗通讯联系人,EＧmail:lishiguiＧsc＠２６３．net)GeneticAnalysisandMappingofaDwarfandHighＧtilleringMutantbf３７０
2、inRiceHUYunＧgao１,２,YANGGuoＧtao２,GUOLianＧan２,QINPeng１,CHENYongＧjun２,LIShiＧgui１,∗(１RiceResearchInstitute,SichuanAgriculturalUniversity,Chengdu６１１１３０,China;２RiceResearchInstitute,SouthwestUniversityofScienceandTechnology,Mianyang６２１０１０,China;∗Correspondingauthor,EＧmail:lishiguiＧsc＠２６３．net)HUYungao,Y
3、ANGGuotao,GUOLianan,etal．GeneticanalysisandmappingofadwarfandhighＧtilleringmutantbf３７０inrice．ChinJRiceSci,２０１５,２９(４):３５７Ｇ３６２．Abstract:AdwarfandhighＧtilleringmutantbf３７０wasobtainedfromtheindicaricevarietyHong’aiBbyneutronradiation．Comparedwiththewildtype,themutantbf３７０wascharacterizedbydwarfplanta
4、ndmoretillers．Thenumberoftillersinbf３７０wasabout２００,whichwas１３timesmorethanthatinthewildtype．Geneticanalysisindicatedthatthisphenotypewascontrolledbyasinglerecessivenucleargene,whichwasmappedtoa３９８kbregionbetweenthemarkerIndel４andIndel１０onthelongarmofchromosome１usingtheF２mappingpopulationgenerated
5、fromthecrossbetweenmutantbf３７０andNipponbare．Sequencinganalysisshoweda６６nucleotidedeletioninthesecondexonofD１０,resultingina２２aminoacidsdeletioninthepredictedRPE６５domainofD１０proteininthebf３７０mutant．Combinedwiththephenotypeofotherd１０mutants,thephenotypeofbf３７０waslikelycausedbya６６nucleotidedeletionin
6、D１０．Keywords:rice;dwarfmutant;highＧtillering;geneticanalysis;mapping胡运高,杨国涛,郭连安,等．水稻矮秆多分蘖突变体bf３７０的遗传分析和基因定位．中国水稻科学,２０１５,２９(４):３５７Ｇ３６２．摘要:通过中子辐射诱变早籼稻品种红矮B,获得矮秆多分蘖突变体bf３７０.该突变体与野生型相比表现为植株矮化,分蘖极多.bf３７０在全生育期内的分蘖数达２００个左右,是野生型分蘖数量的１４倍以上.遗传分析表明该矮秆多分蘖突变体表型受一对隐性核基因控制.利用突变体bf３７０与日本晴杂交构建的F２群体将突变基因定位到第１染色体长臂
7、Indel４与Indel１０之间３９８kb区域内.测序分析发现,与野生型相比突变体该区段内的D１０基因在第２外显子上缺失６６bp碱基,导致D１０蛋白RPE６５结构域２２个氨基酸缺失.结合D１０其他突变体表型推断,bf３７０表型极有可能由D１０突变所致.关键词:水稻;矮秆突变体;多分蘖;遗传分析;基因定位中图分类号:Q３４３?５;S５１１?０３２文献标识码:A文章编号:１００１Ｇ７２１６(２０１５)０４Ｇ０３５７Ｇ０６水稻是人类的主要
