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1、中国水稻科学(ChinJRiceSci),２０１５,２９(６):６０１－６０９http://www．ricesci．cnDOI:１０．３９６９/ji．ssn．１００１Ｇ７２１６．２０１５．０６．００６６０１水稻黄绿叶突变体djyg的遗传分析与基因定位李智强１,２,３朱丹１,２,３王志龙１,２丁波３王国梁１,２,３,∗(１湖南农业大学农学院,长沙４１０１２８;２作物基因工程湖南省重点实验室,长沙４１０１２８;３中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京１００１９３;∗通讯联系人,EＧmail:wang．６２０＠osu．edu)GeneticAn
2、alysisandGeneMappingofaYellowＧGreenLeafMutantdjyginRiceLIZhiＧqiang１,２,３,ZHUDan１,２,３,WANGZhiＧlong１,２,DINGBo３,WANGGuoＧliang１,２,３,∗(１CollegeofAgronomy,HunanAgriculturalUniversity,Changsha４１０１２８,China;２CropGeneEngineeringKeyLaboratoryofHunanProvince,Changsha４１０１２８,China;３StateKeyLaborator
3、yforBiologyofPlantDiseasesandInsectPests,InstituteofPlantProtection,ChineseAcademyofAgriculturalSciences,Beijing１００１９３,China;∗Correspondingauthor,EＧmail:wang．６２０＠osu．edu)LIZhiqiang,ZHUDan,WANGZhilong,etal．GeneticanalysisandgenemappingofayellowＧgreenleafmutantdjyginrice．ChinJRiceSci,２０
4、１５,２９(６):６０１Ｇ６０９．Abstract:AspontaneousyellowＧgreenmutantwasobtainedfromthejaponicaricecultivarDongjininthefield,designatedasdjyg．ThemutantshowedobviousyellowＧgreenleafphenotypeattheseedlingstagebutthechloroticleavesreturnedtogreenaftertheheadingstage．Consistentwiththephenotype,thechlo
5、rophyllbcontentofthemutantwasreducedby５３％,６２％and３６％attheseedling,tilleringandheadingstages,respectively,comparedwiththatofthewildtype(WT)．Atthetilleringstage,weobservedthatthegranasandthylakoidstacksdisplayedamessandloosestructure,aswellaslessdensityinthemutantdjygcomparedwiththeWTund
6、eratransmissionelectronmicroscopy．ThetranscriptionallevelsofPORA,Cab１R,PsbAwerealsodecreasedinthemutantdjyg．GeneticanalysisandmapＧbasedcloningdemonstratedthatthephenotypeofthemutantwascontrolledbyamajorrecessivenucleargene,whichisanalleleofchlorophyllsynthaseYGL１．Themutantdjygcanbeuse
7、dasaphenotypicmarkerinricebreedingprogramsincethemainagronomictraitsofthemutantdjygarenormalcomparedwiththoseoftheWT．Keywords:OryzasativaL．;yellowＧgreenleaf;mutant;mapＧbasedcloning李智强,朱丹,王志龙,等．水稻黄绿叶突变体djyg的遗传分析与基因定位．中国水稻科学,２０１５,２９(６):６０１Ｇ６０９．摘要:在粳稻品种Dongjin大田种植过程中,发现一个黄绿叶自然突变体,命名为djyg
8、.该突变体
