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1、中国水稻科学(ChinJRiceSci),２０１５,２９(３):２４１－２４９http://www．ricesci．cnDOI:１０．３９６９/ji．ssn．１００１Ｇ７２１６．２０１５．０３．００３２４１水稻黄绿叶突变体w３９０的遗传分析和基因定位董青１,２张迎信１,２张振华１,２周全１,２秦亚芝１,２,３王宏１,２程式华１,２曹立勇１,２,∗沈希宏１,２,∗(１中国水稻研究所/国家水稻改良中心,杭州３１１４０１;２浙江省超级稻研究重点实验室,杭州３１１４０１;３四川农业大学水稻研究所/西南作物基因资源与遗传改良教育部重点实验
2、室,成都６１１１３０;∗通讯联系人,EＧmail:xihongshen＠１２６．com;caolycgf＠mail．hz．zj．cn)GeneticAnalysisandGeneMappingofaYellowＧgreenLeafMutantw３９０inRice(OryzasativaL．)DONGQing１,２,ZHANGYingＧxin１,２,ZHANGZhenＧhua１,２,ZHOUQuan１,２,QINYaＧzhi１,２,３,WANGHong１,２,CHENGShiＧhua１,２,CAOLiＧyong１,２,∗,SHEN
3、XiＧhong１,２,∗(１ChinaNationalRiceResearchInstitute/NationalCenterforRiceImprovement,Hangzhou３１１４０１,China;２KeyLaboratoryforZhejiangSuperRiceResearch,Hangzhou３１１４０１,China;３RiceResearchInstitute/KeyLaboratoryofSouthwestCropGeneticResourcesandImprovement,MinistryofEducatio
4、n,SichuanAgriculturalUniversity,Chengdu６１１１３０,China;∗Correspondingauthor,EＧmail:xihongshen＠１２６c．om;caolycgf＠mail．hz．zjc．n)DONGQing,ZHANGYingxin,ZHANGZhenhua,etal．GeneticanalysisandgenemappingofayellowＧgreenleafmutantw３９０inrice(OryzasativaL．)．ChinJRiceSci,２０１５,２９(３):２
5、４１Ｇ２４９．６０Abstract:AyellowＧgreenleafmutant,designatedasw３９０,wasisolatedfromtheprogenyofCoＧγＧtreatedindicacultivarR８０１５．ThemutantexhibitedastableyellowＧgreenleafphenotypeduringthewholelifecycle．Thechlorophyllbwasnotdetectedinw３９０,andthechlorophyllaandcarotenoidscontent
6、sofw３９０werereducedby５０．６％and４４?８％,comparedwiththewildＧtype．Theplantheight,thenumberofpanicleperplant,thenumberofspikeletsperpanicle,thenumberofgrainsperpanicleofw３９０werereducedby１２．０％,２２．３％,１８．５％and２７．６％,respectively．Thetransmissionelectronmicroscopeassaydemonstrated
7、thatthenumberofthylakoidsdecreasedandgranaswerepoorlystackedinthemutant,resultinginunderdevelopmentofchloroplasts．GeneticanalysisshowedthattheyellowＧgreenleafphenotypewascontrolledbyasinglerecessivegene．UsinganF２mappingpopulationderivedfromthecrossofw３９０mutantandNipp
8、onbare,thegenewasdelimitatedtoaregionof７１．８Ｇkbonthelongarmofchromosome１０,whichcontained１５ORFs．Sequenceanalysisrevealedthatthemutant
