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1、作物学报ACTAAGRONOMICASINICA2016,42(5):684689http://zwxb.chinacrops.org/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2016.00684一个水稻黄绿叶突变基因的定位和遗传研究12311,*初志战郭海滨刘小林陈远玲刘耀光12华南农业大学生命科学学院/亚热带农业生物资源保护与利用重点实验室,广东广州510642;华南农业大学公共基础课实验教3学中心,广东广州510642
2、;宜春学院,江西宜春33600060摘要:从粳稻品种日本晴经Co-γ诱变的M1材料中发现一个黄绿叶突变体,其叶片从萌发到三叶前期表现白化,三叶后期开始转为黄绿叶,直到衰老。遗传分析表明,该突变表型受一对隐性核基因控制,将该黄绿叶突变体暂定名为ygl8951。与野生型相比,ygl8951的叶绿素含量与类胡萝卜素含量显著降低。电子显微镜观察表明ygl8951内叶绿体数量明显减少,叶绿体内没有基粒类囊体,只有类似间质类囊体结构。基因表达定量分析表明,突变体中光系统I和光系统II基因表达水平明显下调,核糖体结构基因和质体编码的RNA聚合酶亚基基因表
3、达明显上调。利用ygl8951与籼稻品种黄华占杂交获得的F2分离群体,将该基因定位于水稻第6染色体上的In/Del标记607489与607611之间,物理距离191kb的范围内,通过分析确认该基因为一个新的调控叶色的基因。关键词:水稻;黄绿叶;基因定位;遗传分析GeneticAnalysisandGeneMappingofaYellow-greenLeafMutantinRice12311,*CHUZhi-Zhan,GUOHai-Bin,LIUXiao-Lin,CHENYuan-Ling,andLIUYao-Guang1CollegeofL
4、ifeSciences,SouthChinaAgriculturalUniversity/StateKeyLaboratoryforConservationandUtilizationofSubtropicalAgro-bioresources,2Guangzhou510642,China;CenterofExperimentalTeachingforCommonBasicCourse,SouthChinaAgriculturalUniversity,Guangzhou510642,3China;YichunUniversity,Yichu
5、n336000,China60Abstract:Ayellow-greenleafricemutant,temporarilynamedasygl8951(yellow-greenleaf8951),wasidentifiedfromCoγ-rayradiationmutationinjaponicaricevarietyNipponbare.Themutantshowedalbinophenotypefromgerminationto3-leaf-stage,thenturnedyellow-greenphenotypetillapopt
6、osis.Thecontentsofchlorophyllandcarotenoidwereobviouslydecreasedinygl8951comparedwiththewildtype.Electronmicroscopeobservationshowedthatnogranathylakoidsbutsomestromathyla-koids-likestructureswerefoundinchloroplastofygl8951mutant.Theexpressionlevelsofsomegenesinvolvedinpho
7、tosystemIandphotosystemIIweredramaticallydecreased,whiletheribosomalandRNApolymerasegenesinchloroplastwereincreasedinygl8951mutantcomparedwiththewildtype.Mapping-basedcloningwasusedtoidentifytheygl8951locususinganF2popula-tionfromacrossingbetweenthemutantandHuanghuazhan.Th
8、eresultshowedthatthemutatedlocuswaslocatedina191kbregiononchromosome6,whichwasassumedtobe
