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1、中国水稻科学(ChinJRiceSci),２０１６,３０(３):２３２－２３８http://www．ricesci．cn２３２DOI:１０．１６８１９/j．１００１Ｇ７２１６．２０１６．５１５６水稻迟抽穗突变体dth９的遗传分析与基因定位叶卫军１,２,＃胡时开２,３,＃吴立文２郭龙彪２钱前２,３,∗(１浙江大学农业与生物技术学院,杭州３１００５８;２中国水稻研究所,杭州３１０００６;３中国农业科学院深圳农业基因组研究所,广东深圳５１８１２０;＃共同第一作者;∗通讯联系人,EＧmail:qianqian１８８＠
2、hotmail．com)GeneticAnalysisandGeneMappingofaHeadingＧdelayedMutantdth９inRice(OryzasativaL．)YEWeiＧjun１,２,＃,HUShiＧkai２,３,＃,WULiＧwen２,GUOLongＧbiao２,QIANQian２,３,∗(１CollegeofAgriculture&Biotechnology,ZhejiangUniversity,Hangzhou３１００５８,China;２ChinaNationalRiceResea
3、rchInstiＧtute,Hangzhou３１０００６,China;２AgriculturalGenomicsInstitute,ChineseAcademyofAgriculturalSciences,Shenzhen５１８１２０,China;＃Theseauthorscontributedequallytothiswork;∗Correspondingauthor,EＧmail:qianqian１８８＠hotmailc．om)YEWeijun,HUShikai,WULiwen,etal．Genetica
4、nalysisandgenemappingofaheadingＧdelayedmutantdth９inrice(OryzasativaL．)．ChinJRiceSci,２０１６,３０(３):２３２Ｇ２３８．Abstract:AheadingＧdelayedmutant,dth９(daystoheading９)wasidentifiedfromanethylmethylsulfonate(EMS)Ｇinduced９３Ｇ１１mutantlibrary．Theheadingdateofdth９delayedabou
5、t５０dayscomparedtothewildＧtypeandtherewasnosignificantdifferenceinotheragronomictraits．Geneticanalysisshowedthatthephenotypeofdth９wascontrolledbyasinglerecessivenucleargene．Tomapthisgene,twoF２populationsweregeneratedbycrossingthedth９mutantwithNipponbareorWuy
6、unjing７asmappingpopulations．ByusingSSRmarkersandeightnewdesignedInDelmarkers,DTH９wasnarrowedtoa２４０kbintervalbetweenthemarkersD９Ｇ９andD９Ｇ１７nearthecentromereofchromosome９,therewerenoreportsaboutgenesassociatedwithheadingdateinthisinterval．Inaddition,theexpress
7、ionlevelsofgenesrelatedtoheadingdateweresignificantlydecreasedindth９byquantitativerealＧtimePCRanalysis．Keywords:rice;headingdate;geneticanalysis;genemapping叶卫军,胡时开,吴立文,等．水稻迟抽穗突变体dth９的遗传分析与基因定位．中国水稻科学,２０１６,３０(３):２３２Ｇ２３８．摘要:在EMS诱变的９３Ｇ１１突变体库中筛选到一个稳定遗传的迟抽穗突变体dt
8、h９(daystoheading９).该突变体的抽穗期比野生型延长了５０d左右,其他农艺性状基本无异.遗传分析表明迟抽穗性状受一个隐性核基因控制.以突变体dth９与日本晴和武运粳７号杂交构建的F２分离群体作为定位群体,利用SSR标记和新开发的８个InDel标记,将DTH９定位在第９染色体着丝粒附近D９Ｇ９和D９Ｇ１７之间２４０kb的区间内,该区域尚未发现与抽穗期有关的基因.此外,实时荧光定量PCR结
