褐飞虱体内细菌群落的pcrdgge分析

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1、中国水稻科学(ChinJRiceSci),２０１４,２８(２):２１７－２２２http://www．ricesci．cnDOI:１０．３９６９/ji．ssn．１００１Ｇ７２１６．２０１４．０２．０１５２１７褐飞虱体内细菌群落的PCRＧDGGE分析徐红星１,２郑许松２杨亚军２王新２傅强３叶恭银１,∗吕仲贤２,∗(１浙江大学昆虫科学研究所,杭州３１００２９;２浙江省农业科学院植物保护与微生物研究所,杭州３１００２１;３中国水稻研究所,杭州３１０００６;∗通讯联系人,EＧmail:chu＠zju．edu．cn;luzxmh＠gmail．com)

2、PCRＧDGGEAnalysisoftheBacterialCommunityinDifferentPopulationsofRiceBrownPlanthopper,NilaparvatalugensStålXUHongＧxing１,２,ZHENGXuＧsong２,YANGYaＧjun２,WANGXin２,FUQiang３,YEGongＧyin１∗,LVZhongＧxian２,∗(１InstituteofInsectScience,ZhejiangUniversityHangzhou３１００５８,China;２InstituteofPl

3、antProtectionandMicrobiology,ZhejiangAcademyofAgriculturalSciences,Hangzhou３１００２１,China;３ChinaNationalRiceResearchInstitute,Hangzhou３１０００６,China;∗Correspondingauthor,EＧmail:chu＠zjue．duc．n;luzxmh＠gmailc．om)XUHongxing,ZHANGXusong,YANGYajun,etal．PCRＧDGGEanalysisofthebacteria

4、lcommunityindifferentpopulationsofricebrownplanthopper,NilaparvatalugensStål．ChinJRiceSci,２０１４,２８(２):２１７Ｇ２２２．Abstract:Thebacterialcommunitystructureindifferentvirulentpopulationsofbrownplanthopper(BPH),Nilaparvatalugens(Stål),wasanalyzedbydenaturedgradientgelelectrophores

5、is(DGGE)．TheresultsshowedthatthebacterialcommunityinBPHnymphfromthe１sttothe５thinstarsvariedwithnymphalgrowthanddevelopment,andthebacterialcommunityinthe１stinstarBPHnymphwassimilartothatinadults．NinegeographicBPHpopulationswereobviouslydividedintothreegroupsaccordingtothec

6、lusteranalysisbasedonDGGEfingerprint．ThefirstgroupcamefromthePhilippines,thesecondfromThailandandtheprovincesofHainan,YunnanandZhejiangofChina,andthethirdfromVietnamandtheprovincesofGuangxi,Hunan,andJiangxiofChina．BPHpopulationsonMudgo(withresistantgeneBph１)andASD７(withre

7、sistantgenebph２)havesimilarbacterialcommunity,whiledifferedtothatfedonsusceptiblericevariety,TN１．Keywords:denaturedgradientgelelectrophoresis(DGGE);brownplanthopper(BPH);Nilaparvatalugens;bacterialcommunity;geographicpopulation;virulenttoresistantvarieties徐红星,郑许松,杨亚军,等．褐飞

8、虱体内细菌群落的PCRＧDGGE分析．中国水稻科学,２０１４,２８(２):２１７Ｇ２２２．摘要:应用变性梯度凝胶电泳(denaturedgradientgelelectrophoresis,D