烟叶dna甲基化特征与化学成分的相关性

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1、࿵Ҥ॓࠯/TobaccoScience&Technology࿵Ҥ୪࿐/TobaccoAgronomy2014୍4௹čሹ321௹Ď࿵ွDNAࡊࠎ߄หᆘა߄࿐Ӯٳཌྷܱྟ*ᅵࢭޡđൎᄁ຿đဗᆽཫđ຦ᆽޣđ಩࿐ਅ݌ᇜസ࿵Ҥ॓࿐࿹࣮ჽđ࿵Ҥྛြٳሰ၌Ԯᇗൌဒ൩đ݌ဝܴ೶޵౵ᄉฉКਫ਼550081ᅋေğູૼಒ࿵ွDNAࡊࠎ߄หᆘა߄࿐ӮٳđோྉDArT߄ࠎࡊDNA࿵ोႨ০đ༢ܱٳ༅ਔK326aޣ޽նࣁჭčޣնĎބ቎࿵6ᄝ݌קބྖၬਆ۱൫đ3۱໊࿵ွᄝ҂๝ტൈ௹DNAࡊࠎ߄หᆘބ߄࿐Ӯٳݣਈbࢲݔཁൕđो࿵DNAࡊࠎ߄ඣ௜эၳࢠۚđቋູۚ64%đቋ

2、ູ35.4%đ௜नູ47.61%đэږູ28.6%b௖ᇕࡗࡊࠎ߄ଆൔҵၳቋնđఃՑ൞ളӉ໊bDNAࡊࠎ߄ඣ௜ა12ᇕ໾ᇉսܱ྆࡯ઽࠃྟބ76ᇕᇁཐӮٳཌྷܱٳ༅གྷđDNAࡊࠎ߄აᆃུઽࠃྟބ߄࿐Ӯٳӯࠞཁᇷཌྷܱčpġ0.01Ďđᄝ3۱34ႵٳӮ࿐߄ࠣઽĎ0001.0ġpčܱཌྷᇷཁࠞགྷӯ๝ᇏᇕ௖۱đ҂๝௖ᇕᇏ္ӯགྷԛ൳หၳྟט॥߄࿐໾ᇉbܱ࡯Սğो࿵Ġࡊࠎ߄ĠDArTྉோĠ߄࿐ӮٳĠཌྷܱٳ༅ᇏ๭щᅣ໓Ağ઒്ѓང໓01.572Sğোٳğ1002-0861č2014Ď04-0093-06CorrelationofDNAMethylatio

3、nwithChemicalComponentsinFlue-curedTobacco*ZHAOJiehong,SHIYuewei,YANGZhixiao,WANGZhihong,andRENXueliangMolecularGeneticsKeyLaboratoryofChinaTobacco,GuizhouAcademyofTobaccoScience,Guiyang550081,ChinaAbstract:TofindouttherelationshipbetweenDNAmethylationandchemicalcompon

4、entsinflue-curedtobacco,theDNAmethylationDArTchipofflue-curedtobaccowasusedinanalyzingtheDNAmethylationandchemicalcomponentsinleavessampledfromthreestalkpositionsofcv.K326,HonghuadajinyuanandZunyan6atdifferentdevelopmentstagesinGuidingandXingyi.TheresultsshowedthattheD

5、NAmethylationlevelofflue-curedtobaccovariedwidelyfrom35.4%to64%withtheaveragelevelof47.61%.Thedifferenceofmethylationmodewasbiggerbetweentobaccocultivars,thanthatbetweenstalkpositions.ThecorrelationanalysisofDNAmethylationwiththeactivitiesof12enzymeskeytosubstancemetab

6、olismand76aromaticchemicalcomponentsindicatedthattheircorrelationwasextremelysignificant(p<0.01),therewere34significantlycorrelatedchemicalcomponents/enzymes(p<0.0001)existedinthethreetobaccocultivarsincommon,andspecialregulatedchemicalcompoundswerealsoexistedindiffere

7、ntcultivars.Keywords:Flue-curedtobacco;Methylation;DArTchip;Chemicalcomponent;Correlationanalysisࠎၹቆེջਔള໾ࠎЧ၌Ԯྐ༏đ๙؟ᄖگႋൡၛđ߄ဢ؟ุಕࠣэ๬aቆᇗၹࠎݖ૧хղіၹࠎݖބט॥đथקሢ໾ᇕหᆘbෙಖ๙ݖࠎၹᇗቆࠇ๬эളթߌ࣢ᇏള໾ބ٤ള໾ཽ௧đѩ౏ᆃᇕྩ൥ॖŀ1łэॖࠆྍࠎၹၛൡႋߌ࣢э߄ބཽ௧႕ཙđ֌ࠎၹ๙ݖіܴ၌Ԯ࠺੣༯টbіܴ၌Ԯэၳඣ௜ބଆࣉ߄ჹᇌުႿߌ࣢э߄đᆱ໾ສສ๙ݖDNAࡊࠎ߄aቆൔטࢫሢࠎၹіղٚൔđᆃᇕ

8、эၳࠒ৆ބܿଆ߄Ϣྩ൥ࠇRNAiđॹ෎ؓࠎၹቆࣉྛіܴྩ൥đطᇁ໾ᇕӁളྍі྘đᄝቔ໾ჹჸᄖࢌބ؟П߄ᇏŀ2-3łఏᇗေቔႨbDNAࡊࠎ߄൞ቋᄪགྷࠎၹіܴࠎࣁཛଢğݓࡅ࿵Ҥህછअห೤Ⴊᇉ࿵ွषᇗն॓࠯ህཛज़ีđྩ൥ۚႿᄝթђ௴đ၂ᆭൔ