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1、中国水稻科学(ChinJRiceSci),２０１４,２８(６):５８１－５８８http://www．ricesci．cnDOI:１０．３９６９/ji．ssn．１００１Ｇ７２１６．２０１４．０６．００３５８１极端粒形水稻粒宽基因GW２的序列分析和效应张亚东１,２梁彦丽１,２郑佳２丁丹１,２赵春芳２陈涛２赵庆勇２朱镇２周丽慧２姚姝２赵凌２于新２王才林２,∗(１南京农业大学农学院,南京２１００９５;２江苏省农业科学院粮食作物研究所/江苏省优质水稻工程技术研究中心/国家水稻改良中心南京分中心,南京２１００１４;∗通讯联系人,EＧmail:clwang＠jaas．ac．
2、cn)SequenceAnalysisandEffectofGrainWidthGenesGW２inRiceBasedonExtremeMaterialsinGrainSizeZHANGYaＧdong１,２,LIANGYanＧli１,２,ZHENGJia２,DINGDan１,２,ZHAOChunＧfang２,CHENTao２,ZHAOQingＧyong２,ZHUZhen２,ZHOULiＧhui２,YAOShu２,ZHAOLing２,YUXing２,WANGCaiＧlin２,∗(１CollegeofAgriculture,NanjingAgriculturalU
3、niversity,Nanjing２１００９５,China;２InstituteofFoodCrops,JiangsuAcademyofAgriculturalSciences/JiangsuHighQualityRiceR&DCenter/NanjingBranchofChinaNationalCenterforRiceImprovement,Nanjing２１００１４,China;∗Correspondingauthor,EＧmail:clwang＠jaas．acc．n)ZHANGYadong,LIANGYanli,ZHENGJia,etal．Sequen
4、ceanalysisandeffectofgrainwidthgenesGW２inricebasedonextremematerialsingrainsize．ChinJRiceSci,２０１４,２８(６):５８１Ｇ５８８．Abstract:Grainshapeisoneofthemostimportantinfluencingfactortoricegrainyield．Amongalloftheclonedgrainshapegenes,GW２isanimportantonecontrollinggrainwidth．Weobtainedthesequen
5、cesoftheimportantgrainshapegeneGW２usingcDNAcloningstrategyfromricevarietyTD７０andKasalathanddesignitsfunctionalmarkerbasedongenesequencedifferencetoanalyzegenotypeandphenotypeofrecombinantinbredlines(RILs)fromTD７０/KasalathTD７０cross,someindicarestorerlinesandjaponicaricecultivarsinJia
6、ngsuProvince．GW２shared１００％identitytoWY３GW２and９９．７７％identitytoGW２sequence(GenBank:EF４４７２７５)andthereexistasinglenucleotidemutationatsite１１４withoutchangingaminoacid,asinglenucleotidedeletionatsite３１６whichgenerateterminationcodonatsite３４６,Kasalath３４７and３４８,thustheexpressionofGW２proteins
7、toppedinadvance．ThesequenceofGW２showed９９．９２％identiＧtytoGW２whichisknown,andthereexistsasinglenucleotidechangeatsite１１４withoutthevariationinaminoacid．ComparingwithGW２inTD７０,thegeneinKasalathexpresstheintactproteinwithoutadvancedstop．ForGW２gene,thedCAPSmarkerwasdesignedaccordingtoabase
8、deletionofAinthe３１６
