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1、作物学报ACTAAGRONOMICASINICA2016,42(9):12911297http://zwxb.chinacrops.org/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2016.01291水稻粒形基因GW8的功能标记开发和单体型鉴定*裔传灯王德荣蒋伟李玮成晓俊王颖周勇梁国华顾铭洪扬州大学江苏省作物遗传生理国家重点实验室培育点/粮食作物现代产业技术协同创新中心/教育部植物功能基因组学
2、重点实验室,江苏扬州225009摘要:水稻粒形性状包含粒长、粒宽、粒厚和长宽比,是一类影响水稻产量和品质的重要性状。基于基因GW8序列分析,在该基因启动子区10bp的Indel和第3外显子A/C和T/G的2个变异位点分别开发了功能标记,并将其用于294份水稻微核心种质和2007—2013年江苏省审定的65份粳稻品种的基因型鉴定。研究结果表明这3个变异位点的等位变异对主要的粒形性状有着显著或极显著的影响;其在水稻微核心种质存在的8种单体型对水稻粒形性状有着极显著的影响,其中分别有126、85和58个代表品种的H
3、ap3、Hap6和Hap1是最主要的单体型,单体型Hap7对应水稻籽粒的粒长最长、长宽比最大、千粒重最大;而江苏省审定的粳稻品种中仅发现分别有63个和2个代表品种的单体型是Hap6和Hap2。这些研究结果为水稻产量和品质育种中充分利用基因GW8的优异等位基因或单体型奠定了基础。关键词:水稻;粒形;基因GW8;功能标记;单体型DevelopmentofFunctionalMarkersandIdentificationofHaplotypesforRiceGrainShapeGeneGW8*YIChuan-De
4、ng,WANGDe-Rong,JIANGWei,LiWei,CHENGXiao-Jun,WANGYing,ZHOUYong,LIANGGuo-Hua,andGUMing-HongJiangsuKeyLaboratoryofCropGeneticsandPhysiology,Co-InnovationCenterforModernProductionTechnologyofGrainCrops,KeyLabora-toryofPlantFunctionalGenomicsoftheMinistryofEduca
5、tion,YangzhouUniversity,Yangzhou225009,ChinaAbstract:Ricegrainshapeincludinggrainlength,grainwidth,grainthickness,andgrainlength-to-widthratioisamajorcharac-teraffectingriceyieldandquality.OnthebasisofthealignmentofgeneGW8genomicDNAsequence,threefunctionalm
6、arkersweredevelopedonthe10-bpindelinpromoterregionandtwomissensepolymorphismsites(A/CandT/G)intheexon3,respec-tively.SubsequentlythemarkerswereusedtoidentifythegenotypesofgeneGW8inthe294accessionsofricemini-corecollec-tionand65japonicavarietiescertificatedi
7、nJiangsuprovincefrom2007to2013.Wefoundthattheallelicvariationsofthethreetargetlocihadsignificantorverysignificanteffectsonmosttraitsofgrainshape.OnthebaisofthethreevariationsitesofgeneGW8,eighthaplotypes(combinations)werefoundtohaveverysignificanteffectonth
8、efourgrainshapetraitsinthericemini-corecollection.ThemainhaplotypeswereHap3,Hap6,andHap1,containedin126,85,and58representativevarieties,respectively;Hap7hasthemoststrongesteffectongrainlength,ratioofle
