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1、中国水稻科学(ChinJRiceSci),２０１６,３０(５):４８７－４９２http://www．ricesci．cnDOI:１０．１６８１９/j．１００１Ｇ７２１６．２０１６．６０３８４８７水稻粒宽基因GS５的功能标记开发和单倍型鉴定裔传灯∗王德荣蒋伟李玮成晓俊王颖周勇梁国华顾铭洪(扬州大学江苏省作物遗传生理国家重点实验室培育点/粮食作物现代产业技术协同创新中心/教育部植物功能基因组学重点实验室,江苏扬州２２５００９;∗通讯联系人,EＧmail:cdyi＠yzu．edu．cn)DevelopmentofFunction
2、alMarkersandIdentificationofHaplotypesforRiceGrainWidthGeneGS５YIChuanＧdeng∗,WANGDeＧrong,JIANGWei,LIWei,CHENGXiaoＧjun,WANGYing,ZHOUYong,LIANGGuoＧhua,GUMingＧhong(JiangsuKeyLaboratoryofCropGeneticsandPhysiology/CoＧInnovationCenterforModernProductionTechnologyofGrainC
3、rops/KeyLaboratoryofPlantFunctionalGenomicsoftheMinistryofEducation,YangzhouUniversity,Yangzhou２２５００９,China;∗Correspondingauthor,EＧmail:cdyi＠yzue．duc．n)YIChuandeng,WANGDerong,JIANGWei,etal．Developmentoffunctionalmarkersandidentificationofhaplotypesforricegrainwidt
4、hgeneGS５．ChinJRiceSci,２０１６,３０(５):４８７Ｇ４９２．Abstract:Asamajorcomponentofricegrainshape,thegrainwidthisanimportanttraitwhichiscorrelatedwiththericeyieldandquality．BasedonthealignmentofgeneGS５genomicDNAsequence,twofunctionalmarkersweredevelopedbasedontwomissensepolymor
5、phisms(ACC/CTAintheexon２,A/Cintheexon９,respectively)．Subsequently,themarkerswereusedtoidentifythegenotypesofgeneGS５inthe２９４accessionsofariceminiＧcorecollectionand６５japonicavarietiesreleasedinJiangsuProvincefrom２００７to２０１３．Wefoundthattheallelicvariationsatthetwotarg
6、etlocihadsignificantorextremelysignificantdifferencesinthegrainshapetraits(grainlength,grainwidthandratiooflengthtowidth)．BasedonthetwovariationsitesofgeneGS５,fourhaplotypes(combinations)werefoundinthericeminiＧcorecollection,andhadextremelysignificanteffectongrain
7、width,grainthicknessandratiooflengthtowidthintheindicagroup,andongrainwidthandratiooflengthtowidthinthejaponicagroup,respectively．WhileinjaponicavarietiesreleasedbyJiangsu,onlytwohaplotypes,Hap１(representedby６４varieties)andHap２(representedbyonevariety)werefound．Th
8、eresultswilllayafoundationfortheapplicationoftheusefulalＧlelicvariationsorhaplotypesofgeneGS５tothericeyieldandqualitybreedingprogram．Keywords:rice;grain
