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1、、人_、曰一护“，‘“““不匕夕‘夕亡多才体论文为海洋-02-01,“富含EPA,DHA的水产饵料研究”的一部分。在工作了十四株海洋微藻的总脂含量和脂肪酸组成，筛选出EPA,AA,PUFA含量高或生长迅速的四株红藻，二株隐藻，对它们的塑1胞殖速率，总脂含量，脂肪酸组成进行了不同条件下的比较研究，这些条件包括光照、温度、盐度、氮浓度、磷浓度以及生长期。(})f究结果显示:1.四个门株海洋微藻各具独特的脂肪酸特征:红藻中含有大量的20:4n-6和20:5n-3，其中紫球藻R25含量最高。隐藻的主要脂肪酸是1
2、6:0,18:ln-9,18:3n-3,18:4n-3,20:5n-3,22:5n-3。虫黄藻中18:4n-3含量很少并且不含EPA，主要脂肪酸的是16:0,18:1n-9和22:6n-3aC16和C,，的不饱和脂肪酸是本实验研究的3株绿藻的主要脂肪酸。3.六株微藻的AA,EPA或n3PUFA含量最高的培养条件为:光照温度盐度NaNO,NaH20P=藻种FA收获期Lux℃%。mMMMAA4000293010.2稳后期R6EPA10001560202指数期AA7000151511稳前期R16EPA1000
3、1560202稳前期AA70001521稳后期R25EPA10006082指数期AA700029151稳后期R165EPA1000296020稳前期EPA100015巧42稳前期T11n3PUFAs1000151532指数期EPA1000151542稳前期T13n3PUFAs1000153032指数期3.各藻株收获时达到的最大产量为R6:2015mg/l;R16:3314mg/l;R25:1061mg/l;A165:2050mg/l;Tll:810mg/l;T13:916mg/l。夕/关键词:紫球藻，隐
4、藻，虫黄藻，脂肪酸，总脂t魂厂，乍飞、'AbstractFattyacidcompositionsof14strainsofmarinemicroalgaefromfourtaxonomicdivisionswereexaminedinthispaper.Fourredmicroalgaestrains，twocrytomonadsstrainsrichinPUFA,AA,EPA,DPAorwithhighgrowthrates.werepickedout.Thegrowth,totallipidsco
5、ntentsandfattyacidcompositionofthemicroalgaewereinvestigatedundertheconditionsofdifferentlightillumination,temperature,salinity,nitrateconcentration,phosphorusconcentrationandgrowthphase.Theresultsindicate:1.Eachphylumofmicroalgaewascharacterizedbyaspeci
6、ficfattyacidprofile.Significantconcentrationsof20:4n-6and20:5n-3werepresentintheredmicroalgae,Porl边vridiumsp.withthehighestproportioninRz5(49.8%).Themajorfattyacidsintwocrytomonadswere16:0,18:118:3n-3,18:4n-3and20:5n-3.InZooxanthellamicroadriatica,thefat
7、tyacidcompositionisdifferentfromthatofotherdinoflagellates.物ile么microdriaticacontainslittle18:4n3andnoEPA,themajorcomponentforotherphotosynthesizeare16:0,18:land22:6n-3.16-and18-carbonunsaturatedfattyacidsareimportantcomponentsin3thegreenalgastrainsexami
8、ned.2.TheconditionswhenthesixstrainsofmicroalgaehavethehighestcontentofEPAandAAorn3PUFAwerelistedinthefollowingtable:LightTemp.}Sa编lNaNONaH,OPStrainFAs℃迪GrowthphaseLux--mm29301AA4000---0.2LatestationarR6巧6020EPA1000一-一2Exo
