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1、棉花学报CottonScience2016,28(4):392―398与亚洲棉光籽突变体DPL972中光籽基因紧密连锁的SSR分子标记的开发与应用*冯晓旭袁邹长松袁陆才瑞袁程海亮袁张友平袁MkulamaAPMtawa袁王巧连袁宋国立(中国农业科学院棉花研究所/棉花生物学国家重点实验室袁河南安阳455000)摘要院对棉纤维相关性状基因的克隆及遗传分析可为阐明纤维形成机制奠定良好基础遥以野生型材料DPL971及其光籽突变体DPL972为亲本构建F2群体袁其中光籽与毛籽性状分离比符合3∶1袁表明突变体DPL972中的光籽基因为显性单基因渊将其命名为冤遥结合棉花基因组数据袁合成1567对S
2、SR(Simplesequencere-peat)引物袁覆盖了A组13条染色体袁标记多态性分析和群体连锁分析发现在染色体A08上存在15对与光籽基因连锁的标记袁其中最近的标记为SSR82袁遗传距离为6.6cM遥本实验完成了基因的染色体初步定位袁开发了15个与其连锁的SSR标记袁为下一步图位克隆奠定了基础遥关键词院纤维曰光籽突变体曰曰SSR曰连锁图中图分类号院S562.035文献标志码院A文章编号院1002-7807渊2016冤04-0392-0710.11963/issn.1002-7807.201604011FengXiaoxu,ZouChangsong,LuCairui,Che
3、ngHailiang,ZhangYouping,MkulamaAPMtawa,Wang*Qiaolian,SongGuoli渊455000,冤Thegeneticanalysisandcloningofgenesassociatedwithcottonfibercharacteristicswillnotonlyrevealthemecha-nismoffiberdevelopment,butwillalsoaidmolecularbreeding.Thecotton()mutantDPL972isfuzzless,providingacrucialgermplasmresourc
4、eforcottonresearch.AnF2populationderivedfromacrossbetweenthewild-typeDPL971andthemutantDPL972wasconstructed,andtheresultsoftraitinvestigationfittedasegregationratioof3∶1,whichindicatedthatthefuzzlesstraitwascontrolledbyasingledominantgene(namedhereas).Alinkagemapwasconstructed,whichincluded15s
5、implesequencerepeatmarkersclosetothecandidatetargetgene.ThefuzzlessgenewasinferredtobelocatedonchromosomeA08andwastightlylinkedwithmarkerSSR82atageneticintervalof6.6cM.fiber;fuzzlessmutant;;SSR;linkagemapS562.035A1002-7807渊2016冤04-0392-0710.11963/issn.1002-7807.201604011Cottonisanimportantcomm
6、ercialcropthatisseed-skinduringcottonginning.Furthermore,theplantedworldwide,andisusedasanimportantre-fuzzfrequentlycarriesnumerouspathogens,result-[1-2]sourcefornaturaltextilesandcottonseedoil.inginavarietyofdiseases,e.g.cottonVerticilliumLongfibers,alsocalledlint,canbeeasilywipedoff[3]wilt.I
7、naddition,duringtheseedgerminationpro-fromcottonseeds,whilethefuzzadherestothecess,imbibitionishamperedseverelybythefuzz,2015-12-31FengXiaoxu(1991―),female,Masterdegreecandidate,bbxe2013@163.com;*Corre-spondingauthor,sglzms@163.comTheNa
